| 鸡小肠上皮细胞的分离培养与鉴定 |
| |
| 引用本文： | 李艳,彭春燕,梁榕旺,赵国琦,金晓君.鸡小肠上皮细胞的分离培养与鉴定[J].中国畜牧兽医,2011,38(2):68-71. |
| |
| 作者姓名： | 李艳 彭春燕 梁榕旺 赵国琦 金晓君 |
| |
| 作者单位： | （1.扬州大学动物科学与技术学院，江苏扬州 225009；2.广东博罗县畜牧兽医局，广东博罗 516100） |
| |
| 摘 要： | 选择组织块法分离培养鸡小肠上皮细胞(intestinal epithelial cells，IEC)，采用机械刮除法和相差消化－相差贴壁法纯化细胞，0.05%的Trypsin-EDTA对获得的IEC进行消化传代，免疫细胞化学法鉴定IEC。结果表明，组织块培养法可分离出活性较强的IEC，并获得纯化的上皮细胞；形态学和免疫细胞化学法检测显示获得的细胞表面抗原呈阳性，鉴定为IEC；纯化的IEC可在体外稳定传代。
|
| 关 键 词： | 鸡胚 小肠上皮细胞 体外培养 鉴定 |
Culture of Chicken Intestine Epithelial Cells in vitro and its Characterization |
| |
| LI Yan,PENG Chun-yan,LIANG Rong-wang,ZHAO Guo-qi,JIN Xiao-jun.Culture of Chicken Intestine Epithelial Cells in vitro and its Characterization[J].China Animal Husbandry & Veterinary Medicine,2011,38(2):68-71. |
| |
| Authors: | LI Yan PENG Chun-yan LIANG Rong-wang ZHAO Guo-qi JIN Xiao-jun |
| |
| Institution: | (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2.Animal Husbandry and Veterinary Office of Boluo in Guangdong, Boluo 516100, China) |
| |
| Abstract: | In this study,we selected tissue culture method to isolation chicken intestinal epithelial cells(IEC),using curettage,phase contrast digest and adherence methods to purify cells,with 0.05% Trypsin to digest and passage,immunocytochemical method to identify intestinal epithelial cells.The results showed that hadro-activity intestinal epithelial cells could be dissociated by tissue culture method,and obtain purified intestine epithelium.Morphology and immunocytochemical method showed that intestinal epithelia... |
| |
| Keywords: | chicken embryo intestine epithelial cells in vitro culture characterization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
