| 贵州地区牛源金黄色葡萄球菌分离株16S rRNA分子鉴定及RAPD分型研究 |
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| 引用本文： | 邓海平,蒲万霞,梁剑平,倪春霞,孟晓琴.贵州地区牛源金黄色葡萄球菌分离株16S rRNA分子鉴定及RAPD分型研究[J].中国畜牧兽医,2011,38(1):112-115. |
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| 作者姓名： | 邓海平 蒲万霞 梁剑平 倪春霞 孟晓琴 |
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| 作者单位： | （中国农业科学院兰州畜牧与兽药研究所，中国农业科学院新兽药重点开放实验室，甘肃省新兽药重点实验室，甘肃兰州 730050） |
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| 基金项目： | 国家科技支持计划(2006BAD04A05) |
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| 摘 要： | 利用16S rRNA保守序列对引起贵州地区奶牛乳房炎的金黄色葡萄球菌进行分离鉴定，建立随机多态性扩增体系对金黄色葡萄球菌分离株进行基因分型研究。结果表明，69份奶样中共分离出金黄色葡萄球菌37株，DNA随机多态性扩增（random amplified polymorphic DNA,RAPD）结果显示这37株金黄色葡萄球菌扩增产物在1～7条带之间，产物大小为400～4500 bp。菌株可分为7个基因型，其中Ⅰ型7株（18.9%）、Ⅱ型2株（5.4%）、Ⅲ型2株（5.4%）、Ⅳ型1株（2.7%）、Ⅴ型19株（51.4%）、Ⅵ型1株（2.7%）、Ⅶ型5株（13.5%），Ⅴ型为该地区的流行优势菌群。地理和气候环境对病原菌流行传播的影响是病原菌基因型分布差异的重要原因。
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| 关 键 词： | 奶牛乳房炎 金黄色葡萄球菌 16S rRNA RAPD 基因型 |
Research of Identification by 1 6S rRNA and RAPD Typing on Staphylococcus aureus Isolated from Dairy Cattle in Guizhou |
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| DENG Hai-ping,PU Wan-xia,LIANG Jian-ping,NI Chun-xia,MENG Xiao-qin.Research of Identification by 1 6S rRNA and RAPD Typing on Staphylococcus aureus Isolated from Dairy Cattle in Guizhou[J].China Animal Husbandry & Veterinary Medicine,2011,38(1):112-115. |
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| Authors: | DENG Hai-ping PU Wan-xia LIANG Jian-ping NI Chun-xia MENG Xiao-qin |
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| Institution: | (Key Laboratory of New Animal Drug of Gansu Province,Key Laboratory of New Animal Drug,Lanzhou Institute of Animal Sciences and Veterinary Pharmaceutics，Chinese Academy of Agricultural Sciences，Lanzhou 730050，China) |
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| Abstract: | The 16S rRNA conserved sequence was used for isolation and identification of Staphylococcus aureus(S.aureus) strains causing bovine mastitis in Guizhou,and system of randomly amplified polymorphic DNA(RAPD) was established for research on genetic polymorphism of the S.aureus strains.The results showed that 37 S.aureus strains were isolated and identified from 69 mastitis milk samples.The 37 S.aureus strains amplified out 1 to 7 electrophoresis strips with sizes ranging from 400 to 4500 bp by RAPD.The strain... |
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| Keywords: | bovine mastitis Staphylococcus aureus 16S rRNA RAPD genotype |
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