大白猪和长白猪EPOR基因第4内含子多态性与繁殖性状的关联分析

本研究以大白猪和长白猪为研究对象，以促红细胞生成素受体（erythropoietin receptor，EPOR）基因作为产仔性状的候选基因，采用PCR-SSCP方法来检测EPOR基因第4内含子C＼T突变多态性，研究此位点多态性与猪繁殖性状之间的关联性。结果发现，在长白猪和大白猪中存在AA、AB和BB共3种基因型，在2个群体中处于中度多态。利用SAS 8.0软件采用最小二乘法拟合线性模型，将不同基因型与总产仔数（TNB）、产活仔数（NBA）和初生重（WB）进行了关联分析，结果表明，长白猪初产母猪BB基因型个体的WB显著高于AB、AA基因型个体（P＜0.05）；经产母猪BB基因型个体的TNB显著高于AB基因型个体（P＜0.05）；B等位基因对初产母猪TNB、NBA和WB均表现为正效应。大白猪初产母猪BB基因型个体的TNB、NBA和WB都高于AA型和AB型个体，但差异均不显著（P＞0.05）；经产母猪AB基因型个体的WB显著高于AA基因型个体（P＜0.05）；B等位基因对初产母猪TNB、NBA和WB都表现为正效应。

Genetic Polymorphism of EPOR Gene Intron 4 and Correlation with Reproductive Traits in Large White and Landrace Pigs

A single nucleotide polymorphism (SNP，C＼T) that creates an extra GATA-1 site (T allele) in intron 4 of the swine erythropoietin receptor (EPOR) gene was genotyped use PCR-SSCP method in Large white and Landrace pigs in this study. AA, AB and BB genotypes were found in Landrace and Large white pigs populations, which showed that a moderate polymorphism. Meanwhile, the effect of polymorphism for this loci on total number born (TNB), number born alive (NBA) and born weoght (WB) were analyzed. The results indicated that the individuals of the first parity Landrace pig with BB genotype showed more WB significantly than that with AB and AA genotypes (P<0.05), the individual of multiparous sows with BB genotype showed more TNB significantly than that with AB genotype (P<0.05), and B allele might have the positive effective on TNB, NBA and WB of the first parity sow. While in Large white pig, the TNB，NBA and WB of the first parity sow with BB genotype were more than those with genotype AA and AB, but the results showed no significant difference (P>0.05). The WB of multiparous sows with AB genotype showed more WB significantly than that with AA genotype (P<0.05). B allele might have the positive effective on TNB, NBA and WB of the first parity sow.