| 牛乳中α-酪蛋白完全抗原的合成及多克隆抗体的制备 |
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| 引用本文： | 褚晓红,周玉,李岩松,等.牛乳中α-酪蛋白完全抗原的合成及多克隆抗体的制备[J].中国畜牧兽医,2014,41(2):75-78. |
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| 作者姓名： | 褚晓红 周玉 李岩松 等 |
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| 摘 要： |
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| 关 键 词： | &alpha -酪蛋白 完全抗原 多克隆抗体 |
Synthesis of Complete Antigen and Preparation of Polyclonal Antibody against α-casein in Bovine Milk |
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| CHU Xiao-hong,ZHOU Yu,LI Yan-song,MENG Xing-yu,ZHANG Ying,LU Shi-ying,REN Hong-lin,HU Pan,LIU Zeng-shan,WANG Nan-nan,LI Dan.Synthesis of Complete Antigen and Preparation of Polyclonal Antibody against α-casein in Bovine Milk[J].China Animal Husbandry & Veterinary Medicine,2014,41(2):75-78. |
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| Authors: | CHU Xiao-hong ZHOU Yu LI Yan-song MENG Xing-yu ZHANG Ying LU Shi-ying REN Hong-lin HU Pan LIU Zeng-shan WANG Nan-nan LI Dan |
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| Institution: | （Key Laboratory of Zoonosis Research, Ministry of Education， Institute of Zoonosis， College of Veterinary Medicine, Jilin University，Changchun 130062, China） |
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| Abstract: | In order to gain polyclonal antibody against α-casein, the commercial α-casein were linked with carrier protein BSA and OVA, respectively， as immunogen (α-casein-BSA) and coating antigen (α-casein-OVA). The reaction products were identified by nondenaturing PAGE and SDS-PAGE. New Zealand White rabbits were immunized with the immunogen (α-casein- BSA) to produce polyclonal antibody. The results showed that the titer of the antibody was 1∶256000 and had high cross reactivity with κ-casein. It could be used to detect the total amount of α-casein and κ-casein in milk. |
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| Keywords: | α-casein complete antigen polyclonal antibody |
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