试验性氟中毒对肉鸭肝细胞凋亡的影响

为研究肉鸭氟中毒与肝细胞凋亡的关系及其形态学改变，选用8日龄的天府肉鸭160只，随机分成4组，分别在常规饲料中添加0、350、450和550 mg/kg的氟化钠攻毒，攻毒后每隔8 d，每组扑杀5只，采用末端脱氧核苷酸转移酶 (TdT)介导的dUTP缺口末端标记法 (TUNEL)对试验性氟中毒肝组织标本进行处理和标记，光镜下观测24、40、56日龄肉鸭肝细胞凋亡的百分率和形态学改变。TUNEL检测结果显示，在肉鸭氟中毒模型中，DAB着色的阳性细胞呈凋亡细胞的典型形态特征；肝细胞凋亡的百分率较对照组明显升高。结果表明，肉鸭氟中毒时以肝细胞早期和中期凋亡为主，随着摄氟时间的延长和摄氟剂量的加大，肝细胞由凋亡逐渐发展为坏死。表明过量氟在体内毒性作用和肝细胞凋亡密切相关。

Impact of Experimental Fluorosis on Hepatocyte Apoptosis in Meat Ducks

To study the relationship between fluorosis and hepatocyte apoptosis and morphological changes in meat ducks, 160 8-day-old Tian-fu meat ducks were chosen and randomly divided into four groups,and 0, 350, 450 and 550 mg of sodium fluoride were added to conventional dietary per kg respectively. After challenge five ducks were killed in each group every eight day and TdT-mediated dUTP nick end-labeling (TUNEL) method was used for experimental fluorosis liver tissue handling and labeling. Percentage of hepatocyte apoptosis and morphological changes were detected on the 24th,40th and 56th day with the light microscope. TUNEL results showed that DAB staining positive cells appeared typical morphological characteristics and the percentage of apoptosis cell increased obviously compared with the control group. Fluorosis in meat ducks mainly caused early and middle hepatocyte apoptosis.With time extension and dose increase,sodium fluoride accelerated apoptosis of hepatocyte and developed into necrosis, indicating that toxic effects of excessive fluoride in vivo is closely related to hepatocyte apoptosis.