蒙古羊羊水源干细胞分离培养及其成骨分化研究

试验旨在建立羊水源干细胞系并对其诱导形成成骨细胞的潜能进行研究。在胎牛血清浓度为15%的条件下对羊水源干细胞进行建系,并检测其类胚体形成能力;检测不同代数细胞的干细胞标志基因Oct-4、SH2、SSEA-1、Nanog、HLA-ABC、CD117的表达情况;利用地塞米松、β-甘油磷酸钠、抗坏血酸等因子进行诱导。结果表明,羊水源干细胞在体外可以持续增殖,悬浮培养形成的类胚体表达三胚层相关标记基因,经诱导可形成成骨细胞。试验成功分离了蒙古羊的羊水源干细胞并建系,且其具有向成骨细胞分化的潜能。

Isolation and Culture of Amniotic Fluid-derived Stem Cells from Mongolian Sheep and Induction to Osteoblasts

This study was designed to establish amniotic fluid-derived stem cells line and explore the potential to differentiate into osteoblasts. The amniotic fluid-derived stem cells were cultured under the condition of 15% FBS and detected the embryoid bodies forming ability and investigated the expressions of the stem cell markers (Oct-4,SH2,SSEA-1,Nanog,HLA-ABC,CD117) in different passage cell by RT-PCR. Furthermore,amniotic fluid-derived stem cells were induced by dexamethasone,β-glycerophosphate,ascorbic acid. Amniotic fluid-derived stem cells in vitro were able to expand extensively,form the embryoid body which expressed marker genes of three embryonic germ layers,and differentiate along osteoblasts. The results showed that we successfully isolated the Mongolian sheep amniotic fluid-derived stem cells and established cells line which can be induced to differentiate into osteoblasts.