| 猪圆环病毒2型云南株分离鉴定及ORF2序列分析 |
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| 引用本文： | 李鹏,冯书章,汤会琼,李文贵,柴俊,李作生,张以芳.猪圆环病毒2型云南株分离鉴定及ORF2序列分析[J].中国畜牧兽医,2010,37(5):103-107. |
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| 作者姓名： | 李鹏 冯书章 汤会琼 李文贵 柴俊 李作生 张以芳 |
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| 作者单位： | (1.吉林大学畜牧兽医学院， 长春 130062； 2.云南农业大学动物科学技术学院， 昆明 650201；3.军事医学科学院军事兽医研究所， 长春 130062； 4.云南省蒙自县文澜镇畜牧站， 蒙自 661100) |
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| 基金项目： | 云南省高端科技人才引培工程项目 |
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| 摘 要： | 本研究根据GenBank中已经发表的猪圆环病毒2型基因序列,设计了2对PCV2特异性引物,从疑似断奶仔猪多系统衰竭综合征(postweaning multisystemic wasting syndrome,PMWS)感染病例中检测出3株云南省PCV2流行株,通过ve-ro细胞分离病毒,并用透射电子显微镜观察到了约17nm的病毒样粒子的存在。经同源性比较分析,3个分离株之间核苷酸同源性为99.5%～99.8%,与甘肃省PCV2分离株核苷酸同源性最低(90.7%),浙江省分离株核苷酸同源性最高(99.7%),与南美洲分离株核苷酸同源性最低(92.0%),瑞典分离株核苷酸同源性最高(99.4%),这可能与云南省猪种引进有关。
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| 关 键 词： | 猪圆环病毒2型 分离鉴定 序列分析 |
Isolation and Identification, Squencing and Analyzing of ORF2 Genes of Porcine Circovirus Type 2 from Yunnan Province |
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| LI Peng,FENG Shu-zhang,TANG Hui-qiong,LI Wen-gui,CAI Jun,LI Zuo-sheng,ZHANG Yi-fang.Isolation and Identification, Squencing and Analyzing of ORF2 Genes of Porcine Circovirus Type 2 from Yunnan Province[J].China Animal Husbandry & Veterinary Medicine,2010,37(5):103-107. |
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| Authors: | LI Peng FENG Shu-zhang TANG Hui-qiong LI Wen-gui CAI Jun LI Zuo-sheng ZHANG Yi-fang |
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| Institution: | (1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;2.College of Animal Science and Technology Yunnan Agricultural University, Kunming 650201, China; 3.Institute of Veterinary Science, the Academy of Military Medical Sciences, Changchun 130062, China; 4.Livestock Station of Wenlai Town, Mengzi County, Yunnan Province, Mengzi 661100, China) |
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| Abstract: | According to the complete genome sequences of porcine circovirus type 2 (PCV2) published in GenBank, two pairs of primers were designed. The ORF2 genomes of three PCV2 isolates were amplified from the samples that were similar to those pigs with PMWS, and approximately 17 nm in diameter under electron microscopy. The results of homology showed that nucleotide homology between the three PCV2 isolates was 99.5%-99.8%, and were closely related to the Zhejiang isolate and the Gansu isolate, sharing 99.7% and 90.7%, and displayed 92.0% and 99.4% nucleotide homology with the Sweden isolate and the South America isolate, respectively. |
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| Keywords: | porcine circovirus type 2 isolation and identification sequence analysis |
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