J亚群禽白血病病毒安徽分离株的鉴定及其gp85基因序列分析

从安徽省的黄羽肉鸡和罗曼蛋鸡中各分离鉴定出1株J亚群禽白血病病毒,克隆获得了2条相应的gp85基因序列,并与参考毒株进行序列比对。结果表明,两分离毒株与J亚群参考毒株同源性为82.1%~99.4%,分离毒株之间同源性为85.4%。其中肉鸡分离毒株与J亚群原型毒株HPRS-103同源性为97.1%,与J亚群国内毒株SD09TA04、SDYC02J同源性均为99.4%;蛋鸡分离毒株与HPRS-103的同源性为89.0%,与SD09TA04和SDYC02J同源性仅为88.6%。两分离毒株的gp85氨基酸序列出现突变和缺失,在高变区hr1、hr2变异明显。进化分析进一步表明,2个分离毒株亲缘关系较远,可能来源于不同的原始病毒株。

Idenfication and Sequences Analysis of the gp85 Gene of Subgroup J Avian Leukosis Virus Isolated from Anhui Province

A strain of subgroup J avian leukosis virus(ALV-J) from Mahuang boiler chickens and another strain of ALV-J from Roman layer farms were isolated and identified by serologic test and PCR in Anhui province. The gp85 sequences deduced amino acid of two isolates had 82.1% to 99.4% homology to reference strains from subgroups J. The homology of the two isolates was 85.4%. Strain isolated from the Mahuang boiler chicken had 97.1% homology to strain HPRS-103 from British and 99.4% homology to strain SD09TA04 and SDYC02J ALV-J from China. The Roman layer flock isolated strain had 89.0% homology to HPRS-103 strain and 88.6% homology to strain SD09TA04 and SDYC02J ALV-J. The gp85 deduced amino acid sequences of two isolatess with variation and deletion, hypervariable regions of hr1、hr2 variants were significant. Evolutionary analysis further indicated that two isolates had distant relationship. The results showed that two isolates might come from different subgroups J avian leukosis virus.