| 副猪嗜血杆菌的分离与鉴定 |
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| 引用本文： | 李晓华,杨小燕,郑新添,戴爱玲,李改娜.副猪嗜血杆菌的分离与鉴定[J].中国畜牧兽医,2010,37(2):218-220. |
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| 作者姓名： | 李晓华 杨小燕 郑新添 戴爱玲 李改娜 |
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| 作者单位： | （福建龙岩学院生命科学学院，预防兽医学与生物技术福建省高等学校重点实验室，福建省人畜寄生与病毒性疫病防控工程技术研究中心， 龙岩 364000） |
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| 基金项目： | 福建省科技厅创新平台建设项目(FJ200812) |
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| 摘 要： | 从福建省龙岩市l例临床症状、病理剖检变化疑似副猪嗜血杆菌病的患病猪的关节液中分离到1株革兰氏染色阴性可疑菌株,经卫星现象、生化鉴定等实验室诊断,进一步以副猪嗜血杆菌的16S rRNA基因设计特异性引物进行PCR鉴定,确定该分离菌为副猪嗜血杆菌。药敏试验结果表明,分离株对环丙沙星、卡那霉素、强力霉素、氨苄西林、阿米卡星、庆大霉素药物敏感;对泰妙菌素、诺氟沙星有抵抗力。
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| 关 键 词： | 关键词：副嗜血杆菌 分离鉴定 药敏试验 |
Isolation and Identification of Haemophilus parasuis |
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| LI Xiao-hua,YANG Xiao-yan,ZHENG Xin-tian,DAI Ai-ling,LI Gai-na.Isolation and Identification of Haemophilus parasuis[J].China Animal Husbandry & Veterinary Medicine,2010,37(2):218-220. |
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| Authors: | LI Xiao-hua YANG Xiao-yan ZHENG Xin-tian DAI Ai-ling LI Gai-na |
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| Institution: | (College of Life Sciences of Longyan University, Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Engineering Research Center for the Prevention and Control of Zoonosis, Longyan 364000, China） |
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| Abstract: | A covey of swine whose clinical symptoms looked like Haemophilus parasuis disease were checked by lab technologies.Research as bacteria observation,cultural characteristics and biochemical properties of Haemophilus parasuis were done.According to the sequence of bacterium 16S rRNA,special primers were designed for PCR amplification.The results showed that the strain was tentatively identified as Haemophilus parasuis.The drug sensitive tests showed:the strain was sensitive to Ciprolfloxacin,Kanamycin,Doxycyc... |
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| Keywords: | Haemophilus parasuis isolation and identification drug sensitive test |
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