中国西门塔尔牛血管生成素样蛋白3基因多态性及其与肉质性状的关联性分析

本研究旨在分析血管生成素样蛋白3(angiopoietin-like protein 3,ANGPTL3)基因的单核苷酸多态性(SNPs)及其与中国西门塔尔牛肉质性状的关联性,寻找可用于辅助选择的分子标记。随机选取饲养条件相同的98头中国西门塔尔牛为研究对象,采用PCR-SSCP技术结合DNA测序技术检测ANGPTL3基因的SNP。运用SPSS 19.0软件对ANGPTL3基因的不同基因型与肉质性状进行关联性分析。经测序发现,ANGPTL3基因外显子4中存在1个突变位点G7358C,存在2种基因型:CD、DD。关联性分析结果表明,G7358C突变位点的不同基因型与胴体脂肪覆盖率、眼肌面积、肌内脂肪和大理石花纹差异显著相关(P0.05),CC基因型个体胴体脂肪覆盖率、眼肌面积、肌内脂肪和大理石花纹显著高于CD基因型个体(P0.05)。试验结果表明,G7358C位点的CC基因型为优势基因型,与胴体脂肪覆盖率、眼肌面积、肌内脂肪和大理石花纹等肉用性状有相关性,ANGPTL3基因有望作为分子标记辅助选择的候选基因。

Polymorphism of ANGPTL3 Gene and its Association Analysis with Meat Traits in Chinese Simmental Cattle

The aim of this study was to analyze polymorphism of angiopoietin-like protein 3 (ANGPTL3) gene and its association with meat quality traits in order to find markers in Chinese Simmental cattle. 98 Chinese Simmental cattles under identical feeding condition were selected randomly,the polymorphism of exon 4 of ANGPTL3 gene was analyzed by PCR-SSCP and direct sequencing methods.The association between different genotypes of exon 4 of ANGPTL3 gene and meat traits was analyzed in SPSS 19.0 program.One polymorphic site G7358C which had 2 genotypes of CC and CD was found in ANGPTL3 gene.Association analysis of polymorphisms of ANGPTL3 gene showed that the genotypes of different mutation in ANGPTL3 gene were significantly related to fat coverage of carcass,loin muscle area,marbling and IMF (P<0.05).The fat coverage of carcass,loin muscle area,marbling and IMF of individuals with CC genotype was significantly different from that of individuals with CD genotype (P<0.05).The results indicated that CC genotype was favorable genotype at this SNP locus and associated with fat coverage of carcass,loin muscle area,marbling and IMF,which suggested that ANGPTL3 gene could be studied as a candidate gene for molecular marker assisted selection.