| 实时荧光定量PCR技术的类型、特点与应用 |
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| 引用本文： | 袁亚男,刘文忠.实时荧光定量PCR技术的类型、特点与应用[J].中国畜牧兽医,2008,35(3):27-30. |
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| 作者姓名： | 袁亚男 刘文忠 |
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| 作者单位： | 山西农业大学动物科技学院，太谷 030801 |
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| 基金项目： | 教育部“新世纪优秀人才支持计划”资助项目(NCET-05-0269) |
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| 摘 要： | 实时荧光定量PCR技术是一种利用不同的荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。该技术根据荧光作用方式分为：SYBR荧光染料法、TaqMan技术、分子信标和复合探针等类型，依据其各自优缺点可将各技术方法分别应用于畜牧兽医领域不同方面的科研工作中，以期得到最优的定量检测效率。
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| 关 键 词： | 实时荧光定量PCR技术 荧光共振能量转移 荧光探针 淬灭探针 |
| 文章编号： | 1671-7236(2008)03-0027-04 |
| 修稿时间： | 2007年11月16 |
The Types,Characteristics and Applications of Real-Time Fluoescent Quantitative PCR Techniques |
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| YUAN Ya-nan,LIU Wen-zhong.The Types,Characteristics and Applications of Real-Time Fluoescent Quantitative PCR Techniques[J].China Animal Husbandry & Veterinary Medicine,2008,35(3):27-30. |
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| Authors: | YUAN Ya-nan LIU Wen-zhong |
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| Institution: | College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China |
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| Abstract: | Real time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, and due to its high sensitivity, accuracy, and specificity. This technique can be classified into SYBR GreenⅠ, TaqMan, molecular beacon, complex probes and other methods based on the action modes of fluorescences. Some of the methods'' principles, advantages as well as disadvantages were described in this paper respectively, and they can be applied in different fields of animal and veterinary sciences according to their different characters so as to acquire the best detection efficiency. |
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| Keywords: | real-time fluorescent quantitative PCR fluorescence resonance energy transfer fluorescence probes quenching probes |
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