Complete Mitochondrial DNA D-Loop Sequence and Genetic Diversity of Wuzhishan Pig |
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| Authors: | YU Ping CAO Ting SHI Li-guang LIU Ya-fang ZHAO Chun-ping ZHANG Li-ling HOU Guan-yu |
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| Institution: | 1. College of Agriculture, Hainan University, Haikou 570228, China;2. Tropical Crops Genetic Resource Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China |
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| Abstract: | In order to research the genetic diversity and phylogenies of Wuzhishan pig, 56 complete mtDNA D-Loop were analyzed.55 sequences were amplified by polymerase chain reaction (PCR) and 1 sequence was downloaded from GenBank.The results showed that repetitive sequence "TTATAAAACAC" appeared in the conservative regions of 21 mtDNA D-Loop sequences.13 haplotypes and 14 variable sites were observed in 56 sequences.Haplotype diversity and nucleotide diversity (Hd =0.838 and Pi=0.00334) indicated that Wuzhishan pig had high genetic diversity.Two branches in the phylogenetic tree and Network analysis diagram indicated that there were two maternal origins in Wuzhishan pig.The phylogenetic tree of Wuzhishan pig and other 23 species showed that Wuzhishan pig had close genetic relationship with the pig breeds in the south region of the Yangtze river. |
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| Keywords: | Wuzhishan pig mitochondrial DNA D-Loop genetic diversity |
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