新疆也木勒白羊肌肉生长抑制素基因在不同生长时期的表达研究

试验采用实时荧光定量PCR技术测定也木勒白羊肌肉生长抑制素(myostatin,MSTN)基因在不同生长时期(0～6月龄)的表达量,采用2^－ΔΔCt分析不同生长阶段表达量的差异,旨在掌握也木勒白羊不同生长时期MSTN基因的表达规律,为也木勒白羊肌肉生长性状的选育提供遗传学资料.结果发现,MSTN基因在3月龄也木勒白羊肌肉中的表达量显著低于其他月龄(P<0.05),其余各月龄差异不显著(P>0.05);MSTN在脂肪中的表达量0月龄相对较低,随月龄增长,表达量升高,在5月龄达到最高,后随月龄增长表达量降低.MSTN基因在肌肉和脂肪中的表达量随着月龄增长有一定的规律可循,其表达规律可为也木勒白羊肉用性能的选育提供一定的科学依据.

Expression Levels of MSTN Gene in Different Time of Yemule Sheep

Using quantitative PCR investigate myostatin (MSTN) gene expression levels in different time of Yemnle sheep's muscle and fat (0 to 6 month), using 2^－ΔΔCt analysis of the different expression levels in different time, and aimed to understand the expression levels of Yemule sheep MSTN gene, provided genetic information for the selection of muscle growth traits. The results showed that MSTN gene expression levels in 3 month was significantly higher than the others months (P<0.05), and the others months had no significant differences in the muscle (P>0.05). In the fat , the expression level of MSTN gene present was lower in 0 month, With the growing increase, the expression levels in MSTN gene has increased, and MSTN gene expression levels was the highest in 5 month, then with the growing increase, the expression levels in MSTN gene had decreased. With the growing increase, MSTN gene expression levels had some certain rules, provided scientific basis for the selection of muscle growth traits.