Effect of Recombinant ISG15 Protein from Argali，Bashibai Sheep and their Hybrid Sheep on Sheep Peripheral Blood Lymphocyte Transformation

In order to compare the different effects of recombinant ISG15 proteins in different varieties of sheep on lymphocyte transformation，we compared the effects of five different concentrations recombinant ISG15 proteins of Argali，Bashibai sheep and their hybrid sheep on the sheep peripheral blood lymphocyte transformation.The results showed that when the recombinant ISG15 protein concentrations of Argali sheep were from 10 to 80 μg/mL，D_{570 nm} were significantly higher than PBS control group (P＜0.05); the recombinant ISG15 protein concentrations of Bashibai sheep were from 40 to 80 μg/mL，D_{570 nm} were significantly higher than PBS control group (P＜0.05); while the recombinant ISG15 protein of hybrid sheep，compared with PBS control group，had no significant difference (P＞0.05). In conclusion，certain concentrations of the recombinant ISG15 proteins of Argali and Bashibai sheep could stimulate lymphocyte transformation，with the increase of protein concentrations，the effects of lymphocyte transformation were enhanced，while the recombinant ISG15 protein of hybrid sheep had no significant effect on peripheral blood lymphocyte transformation.