| 副猪嗜血杆菌的分离鉴定及相关特性研究 |
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| 引用本文： | 王雪敏,路明华,陈小玲,路迎迎,张培君,龚玉梅,王宏俊.副猪嗜血杆菌的分离鉴定及相关特性研究[J].中国畜牧兽医,2014,41(5):211-215. |
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| 作者姓名： | 王雪敏 路明华 陈小玲 路迎迎 张培君 龚玉梅 王宏俊 |
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| 基金项目： | 北京市农林科学院创新能力建设项目(KJCX20140410)；国家自然科学基金项目（31001072、31272558）；“863”子课题（2011AA10A210）；河北省教育厅自然科学重点项目（ZH200811）。 |
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| 摘 要： | 本试验从2013年北京地区疑似副猪嗜血杆菌病病料中分离到19株革兰氏阴性短小杆菌，对分离株进行培养特性、荚膜染色、生化特性、血清型分型及PCR鉴定，结果显示分离的19株细菌均为副猪嗜血杆菌（Haemophilus parasuis，Hps），分别属于血清4、5、7、12及13型。对分离株进行药敏试验及致病性试验，结果表明各分离株均有多重耐药性，且各分离株除GS-3外均有较强毒力。
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| 关 键 词： | 副猪嗜血杆菌 分离鉴定 耐药性 毒力 |
Isolation and Identification of Haemophilus parasuisand Correlative Research |
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| WANG Xue-min,LU Ming-hua,CHEN Xiao-ling,LU Ying-ying,ZHANG Pei-jun,GONG Yu-mei,WANG Hong-jun.Isolation and Identification of Haemophilus parasuisand Correlative Research[J].China Animal Husbandry & Veterinary Medicine,2014,41(5):211-215. |
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| Authors: | WANG Xue-min LU Ming-hua CHEN Xiao-ling LU Ying-ying ZHANG Pei-jun GONG Yu-mei WANG Hong-jun |
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| Institution: | (1. College of Agriculture, Hebei University of Engineering, Handan 056021, China; 2. Poultry Diseases Engineering Technology Research Center of Hebei Province, Handan 056021, China; 3. Beijing Municipal Key Laboratory of Animal Disease Prevention and Control Technology, Beijing Institution of Animal Husbandry and Veterinary Medicine, BAAFS, Beijing 100097, China) |
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| Abstract: | In 2013, nineteen small gram-negative bacilli were isolated from pigs with suspected infection of Haemophilus parasuis in Beijing. Isolates were identified as Haemophilus parasuis with the culture characteristics, capsule staining, biochemical method, serotype and PCR identification, and these strains belonged to 4, 5, 7, 12, 13 serotypes. The results of the drug sensitivity test and the pathogenicity test of the nineteen isolates showed that the isolates were multi-drug resistance, and all strains except GS-3 had strong toxicity. |
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