重庆部分羊场沙门氏菌的分离鉴定、耐药表型分析及耐药基因检测

本研究旨在初步了解重庆市羊源沙门氏菌的耐药性及耐药基因流行情况。在5个山羊养殖场采集185份山羊粪便样品，经选择性增菌和PCR鉴定分离沙门氏菌，确定了其血清型，测定了分离菌对28种抗菌药物的敏感性，并检测了喹诺酮耐药决定区（QRDR）的耐药突变位点和质粒介导的喹诺酮耐药（PMQR）、超广谱β-内酰胺酶（ESBL）基因。共分离到羊源沙门氏菌11株，其中10株为德尔卑沙门氏菌。分离的菌株对氨苄西林、头孢唑啉、头孢氨苄、头孢噻肟、四环素和培氟沙星耐药严重；9株菌表现为多重耐药，对3~7类药物耐药。所有菌株均存在QRDR耐药突变且携带bla_TEM基因；7株菌携带1~5种PMQR基因。本研究分离的羊源沙门氏菌对常用抗菌药物整体耐药较为严重，且广泛存在耐药突变和携带耐药基因。

Isolation,Identification,Drug-resistant Phenotype Analysis and Drug Resistant Gene Detection of Salmonella at Goat Farms in Chongqing

The purpose of this research was to acquire the situation of drug resistance and resistance genes prevalence of Salmonella isolated from goats in Chongqing.185 fecal samples were collected from 5 goat farms.Salmonella were isolated by selective enrichment and PCR,and their serotypes were determined.Then,the antimicrobial susceptibility of the isolates to 28 antimicrobial agents was measured.The mutations of quinolone resistance-determining region (QRDR),plasmid-mediated quinolone resistance (PMQR) and extended-spectrum β-lactamases (ESBL) genes were also detected.A total of 11 Salmonella isolates were obtained from goats,and 10 of them were S.Derby.The isolated strains were more resistant to ampicillin,cefazolin,cefalexin,cefotaxime,tetracycline and pefloxacin,and 9 strains showed multi-drug resistant to 3-7 classes of antimicrobial agents.All strains had QRDR resistant mutations and carried bla_TEM gene,whereas,7 strains carried 1-5 PMQR genes.On the whole,Salmonella isolated from goats were resistant to common used antimicrobials.Meanwhile,drug resistant mutations and drug resistant genes were widespread in the isolates.