诱导发情对绒山羊非繁殖季节产羔和血清指标的影响

为研究中草药集中催情和孕马血清促性腺激素（PMSG）外源诱导对非繁殖季节内蒙古绒山羊产羔和血清指标的影响，选取体重相近的内蒙古绒山羊272只，分为中草药试验Ⅰ组（112只）、激素试验Ⅱ组（100只）和对照组（60只），试验Ⅰ组饲喂中草药，每次50 g/只，发情前7 d开始饲喂，每2 d饲喂1次，共3次；试验Ⅱ组海绵栓+PMSG 250 IU/只；对照组正常饲养。在试验结束后第2天早饲前各组选择5只羊颈静脉采血，测定并分析血清中生理生化指标及生殖激素含量的变化。结果显示，试验Ⅰ组和试验Ⅱ组绒山羊的发情率、产羔率和双羔率极显著高于对照组（P<0.01），试验Ⅱ组的产羔率和双羔率极显著高于试验Ⅰ组（P<0.01），发情率显著高于试验Ⅰ组（P<0.05）。试验Ⅱ组绒山羊血清总蛋白（TP）、白蛋白（ALB）、尿素氮（UREA）、谷胱甘肽过氧化物酶（GSH-Px）含量显著高于试验Ⅰ组和对照组（P<0.05），试验Ⅰ组绒山羊丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性显著高于试验Ⅱ组和对照组（P<0.05）；试验Ⅱ组血清促卵泡素（FSH）、雌二醇（E₂）和促黄体素（LH）含量显著高于对照组（P<0.05），孕酮（P₄）、睾酮（T）在三组之间差异均不显著（P>0.05），试验Ⅰ组生殖激素指标与对照组差异均不显著（P>0.05）。对激素和抗氧化酶的相关性分析显示，试验Ⅰ组绒山羊血清P₄含量与GSH-Px含量呈显著负相关（P<0.05），其相关系数为-0.594，试验Ⅱ组绒山羊血清LH含量与GSH-Px含量呈极显著正相关（P<0.01），其相关系数为0.757。本研究结果表明，中草药和激素处理均可极显著促进内蒙古绒山羊在非繁殖季节集中发情，并提高产羔率。激素处理可提高非繁殖季节内蒙古绒山羊对蛋白质的消化利用程度和抗氧化功能，中草药处理可提高血清SOD活性；分析认为GSH-Px与P₄之间存在显著颉颃作用，GSH-Px与LH之间存在极显著协同作用。

Effects of Estrus Induction on Lambing and Serum Index of Cashmere Goats in Non-breeding Season

The aim of the paper was to study Chinese herbal medicine concentration aphrodisiac and PMSG exogenous induction to the non-breeding season goat lambing and serum index of Inner Mongolia cashmere goats.272 Inner Mongolia cashmere goats of similar weight were selected and divided into Chinese herbal medicine test group Ⅰ (112 goats),hormone test group Ⅱ (100 goats) and control group (60 goats).The group Ⅰ was fed Chinese herbal medicine (50 g/goat),starting 7 days before estrus,once every two days;The group Ⅱ sponge plug+PMSG 250 IU per goat,normal feeding of control group.Blood samples were collected from jugular vein of 5 sheep in each group before early feeding on the second day after the end of the experiment,and the changes of physiological,biochemical and reproductive hormones in serum were measured and analyzed.The results showed that the oestrus rate,lambing rate and double lambing rate of the group Ⅰ and the group Ⅱ were significantly higher than that of control group (P<0.01),the lambing rate and double lambing rate of the group Ⅱ were significantly higher than that of the group Ⅰ (P<0.01),and the oestrus rate was significantly higher than that of the group Ⅰ (P<0.05).The contents of TP,ALB,UREA and GSH-Px in the group Ⅱ were significantly higher than those in the group Ⅰ and control group (P<0.05).The content of MDA and SOD activity in the group Ⅰ were significantly higher than those in the group Ⅱ and control group (P<0.05).The contents of FSH,E₂ and LH in the group Ⅱ were significantly higher than those in the control group (P<0.05),and the differences of P₄ and T among the three groups were not significant (P>0.05).The reproductive hormones in the group Ⅰ were higher than those in the control group,but the differences were not significant (P>0.05).Correlation analysis of hormones and antioxidant enzymes showed that the group Ⅰ P₄ was significantly negatively correlated with GSH-Px (P<0.05),with a correlation coefficient of -0.594,while the group Ⅱ LH was significantly positively correlated with GSH-Px (P<0.01),with a correlation coefficient of 0.757.In conclusion,Chinese herbal medicine and hormone treatment could significantly promote the oestrus concentration and increase lambing rate of Inner Mongolia cashmere goats in non-breeding season.Hormone treatment could improve the digestion and utilization of protein and antioxidant function of Inner Mongolia cashmere goats in non-breeding season,and Chinese herbal medicine treatment could increase the activity of serum SOD.It was found that there was significant antagonistic effect between GSH-Px and P₄,and significant promoting effect between GSH-Px and LH.