紫花地丁总黄酮体外抗炎活性研究

试验研究了紫花地丁总黄酮（TFV）对脂多糖（LPS）诱导的小鼠RAW264.7巨噬细胞活力、细胞中炎症介质含量以及相关基因表达的影响，以期探讨其体外抗炎活性的作用。试验采用MTT法筛选出TFV对小鼠RAW264.7巨噬细胞活力具有促进作用的最佳添加浓度；用酶联免疫吸附法（ELISA）检测了TFV对LPS诱导的小鼠RAW264.7巨噬细胞释放到细胞培养液中NO、肿瘤坏死因子α（TNF-α）、白介素1β（IL-1β）、白介素6（IL-6）含量的影响；运用实时荧光定量PCR法检测了TFV对LPS诱导的炎性小鼠RAW264.7巨噬细胞TNF-α、诱导型一氧化氮合酶（iNOS）和环氧合酶2（COX-2）相对表达水平的影响；研究并分析了TFV的体外抗炎活性。试验结果表明，TFV在5~50 μg/mL浓度范围内能提高小鼠RAW264.7巨噬细胞的活力（P<0.05）；与LPS模型组比较，TFV能显著降低LPS诱导的小鼠RAW264.7巨噬细胞产生NO、TNF-α、IL-6、IL-1β的含量，并能显著降低LPS诱导的小鼠RAW264.7巨噬细胞内TNF-α、COX-2等炎症因子的mRNA表达量（P<0.05）。综上，TFV能显著下调LPS诱导的小鼠RAW264.7巨噬细胞IL-1β、IL-6、TNF-α等细胞因子的释放量和下调TNF-α、COX-2 mRNA的表达量，说明抑制促炎性细胞因子基因的表达可能是实现其抗炎作用的原因之一。

Study on Anti-inflammatory Activity of Total Flavonoids from Viola yedoensis in Vitro

To study the effects of total flavonoids from Viola yedoensis(TFV) on the content of inflammatory mediators and the expression of related genes in LPS-induced RAW264.7 cells.Screening of the optimum concentration of TFV for promoting RAW264.7 macrophage viability by MTT method.Then its effects on the release of NO,tumor necrosis factor α(TNF-α),IL-1β,IL-6,TNF-α,iNOS and COX-2 expression on RAW264.7 cells were investigated,thus to study and analyze the in vitro anti-inflammatory activity of TFV.Results showed that TFV could increase RAW264.7 cell viability in the concentration range of 5-50 μg/mL (P<0.05).Comparison with LPS model group,TFV could significantly reduce the production of NO,TNF-α,IL-6 and IL-1β (P<0.05),and could significantly reduce the expression of TNF-α,COX-2 and other inflammatory factors in RAW264.7 cells induced by LPS.TFV could down-regulate the expression of inflammatory factors such as IL-1β,IL-6 and TNF-α,and down-regulate the expression of TNF-α and COX-2,which may be one of the reason for the anti-inflammatory effect of Viola yedoensis.