紫草素抑制炎症促进驴皮伤口愈合的效果

试验旨在探究紫草素凝胶对驴伤口愈合的效果，为皮肤无瘢痕愈合提供参考。用不同浓度的紫草素对体外培养的驴巨噬细胞进行处理，分别检测细胞中TGF-β1、IL-10、TNF-α和IL-6的mRNA与细胞上清液中蛋白的表达水平，以确定紫草素的最佳添加浓度；建立驴皮伤口模型，在伤口表面涂抹紫草素凝胶，拍照、HE染色观察伤口愈合情况，并检测驴皮肤中伤口愈合相关基因和血清的表达水平。结果表明，与对照组相比，添加不同浓度的紫草素均能降低相关炎症因子的表达，其中10 μmol/L紫草素效果最佳。在驴皮伤口上涂抹紫草素凝胶第11天时，紫草素组的伤口收缩率极显著高于对照组（P<0.01）；HE染色发现，第11天时紫草素中的组织逐渐排列整齐；第60天时，紫草素组中表皮层的厚度相对减少；实时荧光定量PCR结果表明，第11天时，紫草素组中TGF-β1、IL-10 mRNA表达极显著升高（P<0.01），TNF-α、IL-6 mRNA的表达极显著降低（P<0.01）；第3天时，紫草素组血清中TGF-β1、IL-10的浓度升高，TNF-α、IL-6浓度极显著降低（P<0.01）；第7天时，紫草素组TGF-β1浓度显著降低（P<0.05），TNF-α浓度极显著降低（P<0.01），且IL-10的浓度显著升高（P<0.05）。综上，与对照组相比，添加不同浓度的紫草素均能降低相关炎症因子的表达，其中10 μmol/L紫草素效果最佳，能促进驴皮伤口的愈合。

Effect of Alkannin on Accelerating Healing Wounds of Donkey Skin by Suppressing Inflammation

The experiment investigated the effect of alkannin gel on wound healing of donkey in order to provide reference for the treatment of skin scars.In vitro cultured donkey macrophages were treated with different concentrations of alkannin,the cells mRNA and supernatant protein expression levels of TGF-β1,IL-10,TNF-α and IL-6 were detected respectively to determine the optimal concentration of alkannin.The skin wound model of donkey was established,and the skin wound was coated with seaweed gel.The wound healing was observed by taking photos and HE staining,and the expression levels of wound healing related genes and serum in the skin of donkey were detected.The results showed that compared with the control group,the addition of alkannin at different concentrations could reduce the expression of related inflammatory factors,among which the 10 μmol/L alkannin group had the best effect.On the 11th day,the wound shrinkage rate of the alkannin group was extremely significant higher than the control group (P<0.01).HE staining showed that the tissues in alkannin were gradually arranged on day 11. At 60th day,the thickness of the superficial cortex in the alkannin group decreased relatively. Real-time quantitative PCR showed that at the 11th day,the expression levels of TGF-β1 and IL-10 mRNA in the alkannin group were extremely significantly increased (P<0.01),while the expression levels of TNF-α and IL-6 mRNA were extremely significantly decreased (P<0.01).At the 3rd day,the serum concentrations of TGF-β1 and IL-10 in the alkannin group were increased,and the concentrations of TNF-α and IL-6 were extremely significantly decreased (P<0.01).At day 7,the concentration of TGF-β1 in the alkannin group was significantly decreased (P<0.05),the concentration of TNF-α was extremely significantly decreased (P<0.01),and the concentration of IL-10 was significantly increased (P<0.05).Therefore,the treatment of donkey skin wound healing with 10 μmol/L alkannin gel had certain anti-inflammatory effect,which promoted the situation of wound healing.