| 蝉抗菌肽基因密码子优化及其毕赤酵母表达载体构建 |
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| 引用本文： | 刘德辉,黄毓茂,徐蕙,焦颖.蝉抗菌肽基因密码子优化及其毕赤酵母表达载体构建[J].中国畜牧兽医,2009,36(10):44-46. |
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| 作者姓名： | 刘德辉 黄毓茂 徐蕙 焦颖 |
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| 作者单位： | (华南农业大学兽医学院， 广州 510642) |
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| 摘 要： | 根据毕赤酵母（Pichia pastoris）密码子的偏嗜性，通过SOEing法合成改造后的抗菌肽Cryptonin基因片段。基因克隆到pGAPZα-A质粒中，获得分泌型重组酵母表达载体pGAPZα-A-Cryptonin。经酶切分析、PCR和测序鉴定证明载体构建成功。表达载体的正确构建为Cryptonin的高效表达和生物学活性研究奠定基础。
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| 关 键 词： | Cryptonin 密码子优化 pGAPZα-A载体 载体构建 |
Codon Optimization of Cicada Antimicrobial Peptide Gene and its Expression Vector Construction in Pichia pastoris |
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| LIU De-hui,HUANG Yu-mao,XU Hui,JIAO Ying.Codon Optimization of Cicada Antimicrobial Peptide Gene and its Expression Vector Construction in Pichia pastoris[J].China Animal Husbandry & Veterinary Medicine,2009,36(10):44-46. |
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| Authors: | LIU De-hui HUANG Yu-mao XU Hui JIAO Ying |
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| Institution: | (College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China) |
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| Abstract: | According to the partiality codon of Pichia pastoris, the antibacterial peptide Cryptonin gene was designed and synthesized. The modified antibacterial peptide gene was cloned into the pGAPZα-A vector to construct the recombinant expression vector pGAPZα-A-Cryptonin. Then the plasmid was identified by restriction enzyme digestion, PCR amplification and sequencing. The successfully constructed plasmid is the background for the studies on high expression and bioactivities of Cryptonin. |
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| Keywords: | Cryptonin codon optimization pGAPZα-A vector vector construction |
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