| 中药王不留行对奶牛乳腺细胞增殖及泌乳的影响 |
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| 引用本文： | 李楠,高学军,关力. 中药王不留行对奶牛乳腺细胞增殖及泌乳的影响[J]. 中国畜牧兽医, 2011, 38(6): 45-48 |
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| 作者姓名： | 李楠 高学军 关力 |
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| 作者单位： | 1. 东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨 150030;2. 黑龙江农业职业技术学院,黑龙江佳木斯 154007 |
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| 基金项目： | 东北农业大学创新团队项目 |
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| 摘 要： | 为确定中药王不留行对奶牛乳腺细胞泌乳功能的影响,以王不留行为原料,通过水浸提得到王不留行提取液,测出王不留行提取液中增乳活性物质邻苯二甲酸二丁酯含量,设置不同浓度中药提取液进行细胞试验,观察其对细胞泌乳的影响,筛选出增乳效力最显著的药物浓度。结果2 mg/mL(生药浓度)药物组增乳效果最显著,提示中药王不留行可直接作用于奶牛乳腺上皮细胞促进泌乳。
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| 关 键 词： | 王不留行 乳腺上皮细胞 泌乳 |
| 收稿时间： | 2010-11-17 |
Effects of Semen vaccariae on the Proliferation and Lactation Fuction of Bovine Mammary Gland Epithelial Cell |
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| LI Nan,GAO Xue-jun,GUAN Li. Effects of Semen vaccariae on the Proliferation and Lactation Fuction of Bovine Mammary Gland Epithelial Cell[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(6): 45-48 |
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| Authors: | LI Nan GAO Xue-jun GUAN Li |
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| Affiliation: | 1. Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China;2. Heilongjiang Agricultural Vocationl and Technical College,Jiamusi 154007,China |
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| Abstract: | To determine the effects of Chinese traditional medicine Semen vaccariae on the proliferation and lactation fuction of bovine mammary gland epithelial cell,the water decoction was obtained from Semen vaccariae.The content of Semen vaccariae active material,dibutyl phthalate (DBP) was detected,and different concentration gradients of the drug extraction were observed for their influence on cell lactation. The most effective drug concentration was screened out for lactogenesis.The results showed that 2 mg/mL(crude drug concentration) proup was the most effective concentration for lactogenesis of bovine mammary gland epithelial cell. The results suggest that Semen vaccariae can act directly on bovine mammary gland epithelial cell for lactogenesis. |
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| Keywords: | Semen vaccariae mammary gland epithelial cell lactation |
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