花艽-6抗炎作用及其机理研究

本试验旨在研究和探讨抗类风湿性关节炎(rheumatoid arthritis,RA)药物花艽-6的抗炎作用及其抗炎机理。采用二甲苯致小鼠耳肿胀法、醋酸致小鼠毛细血管通透性增高法、甲醛和蛋清致小鼠足跖肿胀法,以及棉球致小鼠肉芽组织增生法分别研究了该药物的抗急性、亚急性和慢性炎症的作用。通过复制佐剂性关节炎(adjuvant arthritis,AA)大鼠模型研究花艽-6对其血清中IL-1β、IL-6和TNF-α含量的影响。花艽-6对小鼠二甲苯所致的耳肿胀、醋酸所致的腹腔毛细血管通透性增高、甲醛和蛋清所致的足跖肿胀和棉花所致的肉芽组织增生均具有显著或极显著的抑制作用。花艽-6具有抗炎作用,可显著降低AA大鼠血清中IL-1β、IL-6和TNF-α的表达,对RA具有治疗作用。

Study on the Anti-inflammatory Effect and Mechanism of Huajiao-6 Decoction

To study the anti-inflammatory effect and mechanism of Huajiao-6 decoction, used the xylene-induced inflammation,paw swelling,acetic acid to make the permeabitity of blood capillaries and cotton pellet-induced granuloma method in mice.Determined the ear swelling,paw edema caused by egg white and formaldehyde and cotton pellet induced granuloma after administration.The adjuvant arthritis(AA) rat model was made, then,the activity of IL-1β,IL-6 and TNF-α of AA rat were determined by the method of ELISA. Huajiao-6 decoction significantly inhibited the acute inflammation in mice induced by xylene as well as the acetic acid to make the permeabitity of blood capillaries,subacute inflammation model caused by egg white and formaldehyde and the chronic inflammation model aroused by intradermal implantation cotton pellet. Huajiao-6 had significant inhibition on inflammation, the content of IL-1β,IL-6 and TNF-α in serum of AA rat were significantly reduced by Huajiao-6 decoction. The results showed that Huajiao-6 decoction had the inhibitory effect on the activity of inflammatory mediums and responds, it could be used to treat RA.