| 不同因素对绵羊卵母细胞体外受精效果的影响 |
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| 引用本文： | 汪立芹,赵云程,陈童,陈世彬,左北瑶,彭夏雨,黄俊成,郭志勤.不同因素对绵羊卵母细胞体外受精效果的影响[J].中国畜牧兽医,2010,37(8):84-86. |
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| 作者姓名： | 汪立芹 赵云程 陈童 陈世彬 左北瑶 彭夏雨 黄俊成 郭志勤 |
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| 作者单位： | （1.新疆动物生物技术重点开放实验室，农业部草食家畜繁育生物技术重点开放实验室，乌鲁木齐 830000; 2.新疆农业大学动物医学学院，乌鲁木齐 830052；3.南京农业大学动物胚胎工程技术中心，南京 210095；4.石河子大学动物科技学院，石河子 832000） |
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| 基金项目： | 国家支撑计划，新疆自治区科技攻关，新疆自治区自然科学基金，新疆自治区科技计划项目 |
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| 摘 要： | 本试验研究了发情羊血清、不同个体的冷冻精液和新鲜精液、精子密度、受精时颗粒细胞的有无及不同培养液对绵羊体外受精效果的影响。结果表明,发情3d的血清与发情当天的血清相比,对卵裂率和囊胚率有极显著的影响(P0.01);不同个体的冷冻精子对卵裂率和囊胚率都有显著的影响(P0.05),但鲜精和冷冻精子的受精效果与个体有关;精子密度在2×106～6×106个/mL之间对受精效果没有影响(P0.05);颗粒细胞对受精的作用不明显(P0.05);不同的培养液对受精效果没有显著影响(P0.05)。因此,选用好的公羊个体精子,用发情3d的发情羊血清能显著提高受精效果。
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| 关 键 词： | 绵羊 体外受精 发情血清 卵裂率 囊胚率 |
The Effect of Different Factors on the Development of Embryos from in vitro Fertilization of Sheep |
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| WANG Li-qin,ZHAO Yun-cheng,CHEN Tong,CHEN Shi-bin,ZUO Bei-yao,PENG Xia-yu,HUANG Jun-cheng,GUO Zhi-qin.The Effect of Different Factors on the Development of Embryos from in vitro Fertilization of Sheep[J].China Animal Husbandry & Veterinary Medicine,2010,37(8):84-86. |
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| Authors: | WANG Li-qin ZHAO Yun-cheng CHEN Tong CHEN Shi-bin ZUO Bei-yao PENG Xia-yu HUANG Jun-cheng GUO Zhi-qin |
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| Institution: | (1. Key Laboratory of Animal Biotechnology of Xinjiang，Key Laboratory of Grass Livestock Reproduction ＆Breed Biotechnology Ministry of Agriculture,Urumqi 830000, China;2.College of Animal and Veterinary,Xinjiang Agricultural University,Urumqi 830052,China;3. Center of Animal Embryo Engineering and Technology, Nanjing Agricultural University, Nanjing 210095,China;4.College of Animal Science and Technology， Shihezi University ,Shihezi 832000,China) |
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| Abstract: | The study was conducted to determine the effect of diffent heat serum, diffent sheep individuals,sperm density,cumulus’effect and different culture media on the development of embryos from in vitro fertilization.The results showed that heat three days serum affect greatly the cleavage rate (CR) and the blastocyst rate (BR)（P＜0.01）;and the different sperm how ever they are freezed or fresh have significant affect on CR and BR. No significant differences in CR and BR were found both sperm densities of 2×106 to 6×106 sperms/mL.The cumulus of oocyte have no effect on IVF;there were no different effect on BR and CR of the culture media of SOF and CRLaa. |
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| Keywords: | sheep IVF heat serum cleavage rate blastocyst rate |
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