| 饲料中3种四环素类药物的高效毛细管电泳检测方法 |
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| 引用本文： | 饶钦雄,任琳琳,童敬,郭平,李海燕,丁双阳.饲料中3种四环素类药物的高效毛细管电泳检测方法[J].中国畜牧兽医,2009,36(9):74-78. |
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| 作者姓名： | 饶钦雄 任琳琳 童敬 郭平 李海燕 丁双阳 |
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| 作者单位： | (1.上海农业科学院农产品质量标准与检测技术研究所, 上海 201106; 2.中国农业大学 动物医学院/国家兽药残留基准实验室， 北京 100193; 3. 中华人民共和国江西出入境检验检疫局, 南昌 330002) |
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| 基金项目： | 国家"十一五"科技支撑计划项目资助 |
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| 摘 要： | 作者建立了鸡饲料中四环素、土霉素和多西环素的高效毛细管电泳检测方法。3种药物在40 mmol/L磷酸氢二钠—20 mmol/L柠檬酸(pH 2.6)的电泳缓冲液、30 kV的分离电压、长度64.5 cm、内径75 μm的毛细管内得到完全分离。在1～80 mg/kg范围内3种药物线性良好，R2>0.994；配合饲料和预混料中3种药物的定量限分别为2和4 mg/kg；添加回收试验回收率>80%，方法的日内变异系数和日间变异系数均<10%。
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| 关 键 词： | 四环素 土霉素 多西环素 高效毛细管电泳 饲料 |
Determination of Tetracyclines in Fowl Feeds and Premix by High Performance Capillary Electrophoresis |
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| RAO Qin-xiong,REN Lin-lin,TONG Jing,GUO Ping,LI Hai-yan,DING Shuang-yang.Determination of Tetracyclines in Fowl Feeds and Premix by High Performance Capillary Electrophoresis[J].China Animal Husbandry & Veterinary Medicine,2009,36(9):74-78. |
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| Authors: | RAO Qin-xiong REN Lin-lin TONG Jing GUO Ping LI Hai-yan DING Shuang-yang |
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| Institution: | (1.Institute for Agr-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201106， China； 2.National Reference Laboratories for Veterinary Drug Residue, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193， China； 3.Jiangxi Entry-Exit and Inspection and Quarantine, Nanchang 330002， China) |
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| Abstract: | A high performance capillaty electrophoresis (HPCE) method has been established for the separation of tetracycline1, oxytetracycline and doxycycline in fowl feeds and premix. Separation was carried out in a 64.5 cm×75 μm I.D. fused-silica uncoated capillary in 40 mmol/L Na2HPO4-20 mmol/L Citric acid buffer (pH 2.6) with 30 kV. The linear of the melamine was 1 mg/kg to 80 mg/kg, and correlation index was higher than 0.994. Limit of quantitations of feeds and premix were 2 mg/kg and 4 mg/kg, respectively. The average recoveries were higher than 80% in the spiked range from the concentration of 2 mg/kg to 80 mg/kg, and RSDs were lower than 10%. |
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| Keywords: | tetracyclinel oxytetracycline doxycycline HPCE feeds |
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