不同光照下褪黑素变化对鸡骨髓内c-fos原癌基因表达的影响

为了找出褪黑素分泌变化与c-fos原癌基因表达之间的关系，本研究控制不同光照时间饲养1日龄雏鸡50只，取骨髓组织，经免疫组化试验，结果显示：①单核细胞系胞浆出现阳性信号；巨核细胞系、粒细胞系和淋巴细胞系胞核出现阳性信号；红细胞系无阳性信号；②随着光照时间的延长，c-fos原癌基因的表达量呈增长趋势。结果表明，c-fos与骨髓细胞的细胞增殖和分化相关。褪黑素的增高能够显著抑制c-fos原癌基因的表达，且褪黑素的减少能够刺激c-fos的表达。

Effects of Melatonin on c-fos Oncogene Expression of the Bone Marrow of Chicken in Different Illumination Conditions

In order to find out the relationship between the changes of melatonin secretion and expression of c-fos oncogene, in this study, we have one day-old chicks by controlling different illumination conditions, then take out 1 cm bone marrow, immunohistochemical analysis showed that：①positive signal in the cytoplasm of monocytic series; positive signal in the cell nuclei of megakaryocytic series, granulocytic series and lymphocyteic series; no positive signal in erythrocyte. ②With the extension of illumination time, the expression of c-fos had a growth trend. The results showed that c-fos is related to cell proliferation and differentiation of bone marrow cells. The increase of melatonin can significantly inhibit c-fos oncogene expression and the reduction of melatonin can stimulate c-fos expression. According to the results of this experiment can speculate that melatonin can impact on proliferation and differentiation of bone marrow cells by regulating the expression of c-fos.