UCP1基因变异与绵羊生长及胴体性状的关联分析

试验旨在探讨解耦联蛋白1（UCP1）基因核苷酸变异对绵羊生长性状的性别差异和胴体性状的影响，以期能够筛选出可以提升绵羊生长及胴体性状的核苷酸变异，为提高绵羊相关重要经济性状的分子遗传标记提供材料。以9个绵羊品种为研究对象，用PCR-SSCP方法检测不同性别绵羊中UCP1基因内含子5区和外显子6区变异。利用Minitab 16.0软件中一般线性模型分析内含子5区等位基因与不同性别绵羊生长性状、公羔胴体性状的关联性。结果显示，绵羊UCP1基因内含子5区和外显子6区共检测到8个核苷酸变异，其中位点c.910 G/A突变导致p.Ala304Thr氨基酸变异。生长性状关联分析结果表明，内含子5区等位基因对绵羊生长性状的影响存在性别特异性，母羔中携带等位基因A₁的群体较缺失群体具有较低的初生重（P<0.05），公羔中携带等位基因C₁的群体较缺失群体具有较高的断尾重（P<0.05），未发现其他等位基因与羔羊的生长性状存在性别特异性。胴体性状关联分析结果表明，携带等位基因A₁的群体具有较低后腿瘦肉量、腰部瘦肉量和较高的肩部瘦肉比例（P<0.05），携带等位基因C₁的群体具有较低的后腿瘦肉比例（P<0.05），其他胴体性状均没有发现与等位基因存在显著相关，基因型分析结果与等位基因分析结果一致。结果提示，UCP1基因对绵羊的生长性状影响具有性别特异性，且携带等位基因A₁的公羔群体具有较低的胴体生产性状，为提高公羔胴体生产性能提供了理论依据。

Association Analysis of UCP1 Gene Variation with Growth and Carcass Traits in Ovine

This study was aimed to analyze the effect of UCP1 gene variation on growth and carcass traits in ovine,so as to screen out the nucleotide variation that could improve growth and carcass traits in ovine and provide materials for molecular genetic markers of important economic traits related to ovine.The mutations in intron 5 and exon 6 regions of ovine UCP1 gene were detected by PCR-SSCP in 9 ovine populations,and the relationships between mutations in growth in male and female lambs and carcass traits in male lambs were investigated using General Linear Models (GLMs) by Minitab 16.0 software.The results showed that 8 SNPs were detected in intron 5 and exon 6 regions of ovine UCP1 gene,the nucleotide substitution c.910 G/A in exon 6 resulted in amino acid change (p.Ala304Thr).The results of correlation analysis of growth traits showed that the allele in intron-5 region of ovine UCP1 gene had different effects on growth traits of male and female lambs.The presence of allele A₁ was associated with decreased birth weight in female lambs (P<0.05),and the presence of allele C₁ was associated with increased tailing weight in male lambs (P<0.05),no associations were detected between the other allele and growth traits in male and female lambs.The results of correlation analysis of carcass traits showed that the presence of allele A₁ was associated with decreased leg yield,loin yield and increased proportion of should yield (P<0.05),and the presence of allele C₁ was associated with decreased proportion of should yield (P<0.05),no associations were detected between the other allele and carcass traits in male lambs,and the genotype analysis results was consistent with allele analysis.The results indicated that the effect of ovine UCP1 gene on growth traits was gender-specific,and allele A₁ had lower carcass production traits in male lambs,which provided a theoretical basis for improving carcass production in male lambs.