SH2B1基因及miR-276-3p对猪背部脂肪沉积的影响

本试验旨在研究SH2B衔接因子蛋白1（SH2B adaptor protein 1，SH2B1）基因在猪不同组织和生长发育各阶段背部脂肪中的表达情况，预测调控该基因的miR-276-3p对猪背部脂肪表达的影响。应用实时荧光定量PCR技术检测SH2B1基因在猪脂肪、下丘脑等6种组织，以及在30、60、90、120和180 d猪背部脂肪组织中的相对表达量。靶标预测SH2B1基因的调控miRNA，并通过实时荧光定量PCR检测miR-276-3p对该基因的调控作用。结果显示，SH2B1基因在猪的6种组织中均有表达，且在脂肪组织中表达量最高，在肌肉组织中表达量最低。在猪生长发育各阶段背部脂肪中SH2B1基因均有表达，在前期（30和60 d）表达量较低，在中、后期（90、120和180 d）持续高表达，且显著高于前期表达量（P<0.05）。高、低背膘厚组背部脂肪中miR-276-3p与SH2B1基因均呈差异表达，且两者表达呈相反趋势，miR-276-3p在高背膘厚组中的表达量显著低于低背膘厚组（P<0.05），而SH2B1基因在高背膘厚组中的表达量却显著高于低背膘厚组（P<0.05）。miR-276-3p可通过靶向负调控SH2B1基因，影响猪背部脂肪的沉积。本试验结果为进一步深入研究猪背部脂肪沉积和背膘厚差异的分子机制提供参考。

Effects of SH2B1 Gene and miR-276-3p on Backfat Deposition in Pigs

This study was aimed to investigate the expression of SH2B1 gene in the different tissues and backfat at various growth stages of pigs,and predict the effect of miR-276-3p which regulated SH2B1 gene on the expression of backfat in pigs.Real-time quantitative PCR was used to detect the relative expression of SH2B1 gene of six tissues in pigs,and in backfat of pigs at 30,60,90,120 and 180 d.The target regulation miRNA of SH2B1 gene was predicted and the regulation of SH2B1 gene by miR-276-3p was detected by Real-time quantitative PCR.The results showed that SH2B1 gene was expressed in all six tissues,with the highest expression in fat and the lowest expression in muscle.The expression of SH2B1 gene was expressed at various stages of growth and development in pigs.The expression level was low at the early stage (30 and 60 d) and high at the middle and late stages (90,120 and 180 d).The expression of SH2B1 gene was significantly higher at the middle and late stages than that at the early stage (P<0.05).The relative expression of miRNA-276-3p and SH2B1 gene in backfat tissue of pigs that in high or low backfat thickness group (HBFT or LBFT) was detected by Real-time quantitative PCR.The results showed that miRNA-276-3p and SH2B1 gene were differentially expressed in backfat tissue of pigs.The expression of miRNA-276-3p in high backfat thickness group was significantly lower than that in low backfat thickness group (P<0.05),while the expression of SH2B1 gene in high backfat thickness group was significantly higher than that in low backfat thickness group (P<0.05).miRNA-276-3p negatively regulated SH2B1 gene and affected the fat deposition on the back of pigs.This results provided a reference for further studying the molecular mechanism of fat deposition and backfat thickness difference in pigs.