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| 猪A群轮状病毒BJ株的分离与鉴定 | |
| 引用本文： | 原霖,韩焘,倪建强,亢文华,汪葆玥,李晓霞,陈亚娜,翟新验,沈建忠.猪A群轮状病毒BJ株的分离与鉴定[J].中国畜牧兽医,2016,43(12):3306-3313. |
| 作者姓名： | 原霖 韩焘 倪建强 亢文华 汪葆玥 李晓霞 陈亚娜 翟新验 沈建忠 |
| 作者单位： | 1. 中国农业大学动物医学院, 北京 100193; 2. 中国动物疫病预防控制中心, OIE猪繁殖与呼吸综合征参考实验室, 北京 102618 |
| 基金项目： | 科技部科技基础性工作专项--动物及动物产品质量安全检测标准物质研制（2013FY113300-6） |
| 摘 要： | 轮状病毒是引起幼龄动物和儿童病毒性腹泻的主要病原，轮状病毒的分离鉴定为该病的流行病学调查和分子生物学特性研究奠定了基础。本研究采集北京某猪场腹泻发病仔猪肠内容物，将其接种MA104细胞，分离得到一株能产生明显细胞病变的病毒。对分离毒株进行胶体金、实时荧光定量RT-PCR和免疫荧光等方法鉴定，并对分离毒株的VP4、VP6和VP7基因进行测序及序列分析。结果表明，分离毒株为猪A群轮状病毒。按照A群轮状病毒的最新分类方法，确定该分离株VP4、VP6和VP7基因的基因型分别为P13]型、I5型和G11型。因此，将该分离株命名为Rotavirus A pig/China/BJ/2015/G11P13]。 |
| 关 键 词： | 猪轮状病毒 分离 鉴定 |
| 收稿时间： | 2016-05-11 |
Isolation and Identification of Porcine Group A Rotavirus BJ Strain |
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| YUAN Lin,HAN Tao,NI Jian-qiang,KANG Wen-hua,WANG Bao-yue,LI Xiao-xia,CHEN Ya-na,ZHAI Xin-yan,SHEN Jian-zhong.Isolation and Identification of Porcine Group A Rotavirus BJ Strain[J].China Animal Husbandry & Veterinary Medicine,2016,43(12):3306-3313. | |
| Authors: | YUAN Lin HAN Tao NI Jian-qiang KANG Wen-hua WANG Bao-yue LI Xiao-xia CHEN Ya-na ZHAI Xin-yan SHEN Jian-zhong |
| Institution: | 1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; 2. OIE Reference Laboratory for Porcine Reproductive and Respiratory Syndrome, China Animal Disease Prevention and Control Center, Beijing 102618, China |
| Abstract: | Rotavirus infections are a major cause of viral diarrheas in young animals and children. Isolation and identification of rotavirus make a contribution to epidemiological survey and molecular biology study.A strain of porcine rotavirus was isolated in MA104 cell cultures from the intestinal contents of piglets with diarrhea in Beijing.The virus was identified to be porcine rotavirus by immunochromatography strip test, Real-time RT-PCR, immunofluorescence test and sequencing analysis.According to the sequence analysis, the virus was classified as group A porcine rotavirus, the genotype of VP4, VP6 and VP7 genes belonged to P13], I5 and G11, respectively.The virus was designated Rotavirus A pig/China/BJ/2015/G11P13]. |
| Keywords: | porcine rotavirus isolation identification |
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