引发仔猪水样腹泻的多重耐药、高致病性大肠埃希菌的分离与鉴定

近来,长春地区多个养猪场发生高致病性、高死亡率的仔猪水样腹泻,腹泻仔猪经多种药物反复治疗,均无疗效。试验从发病仔猪肠壁内膜分离到2株大肠埃希菌(Escherichia coli),经鉴定,分离菌株为多耐药、高致病性菌株。将分离株进行生化试验及其16S rRNA序列的克隆、测序,并与GenBank中的序列进行对比,结果显示,分离菌株16S rRNA与GenBank登录的大肠埃希菌序列同源性达99%。动物回归试验结果显示,该大肠埃希菌能引起仔猪水样腹泻。药敏试验结果显示,常用治疗大肠杆菌病的药物如庆大霉素、氧氟沙星、环丙沙星等均对其无效。

Separation and Identification of Multi-drug Resistant and High Pathogenic Escherichia coli Causing Piglets Watery Diarrhea

Piglets watery diarrhea with high pathogenity and high mortality rate happened in many farms in Changchun area, the diarrhea piglets repeatedly tried a variety of medications,but no effect came out. From the onset of piglets intestinal wall lining,we had separated and identified some drugresistant and highly pathogenic E.coli strains.After the biochemical test,and cloning and sequencing of 16S rRNA of the strains,we compared them with the sequences in GenBank,the isolated strains had 99% homology with the 16S rRNA of E.coli sequence. After animal testing, we determined that this kind of E.coli could cause piglets watery diarrhea. Drug sensitivity test results showed that drugs commonly used in E.coli disease as treatments such as gentamicin,ofloxacin and ciprofloxacin were invalid.