氟对雄性小鼠生长发育及性腺中氟含量的影响

本试验旨在研究氟对性成熟期雄性小鼠生长发育及性腺中氟含量的影响。将40只健康昆明雄性小鼠随机分为4组,即低、中、高3个染氟组和对照组,其中染氟组分别饮水摄入氟化钠30、70和150 mg/L,对照组饮用蒸馏水,染氟期间,观察动物的一般状况,每周记录小鼠体重,49 d后,收集血液、股骨、睾丸、附睾和精囊腺,测定其氟含量。与对照组相比,各染氟组体重均无显著差异(P＞0.05),高氟组小鼠体增重显著低于对照组(P＜0.05);各染氟组股骨氟含量均显著高于对照组(P＜0.05),睾丸和附睾中氟含量随摄氟剂量的增加呈剂量依赖性,且高氟组显著升高(P＜0.05),血清与精囊腺中氟含量与对照组相比无显著差异(P＞0.05)。结果表明,高氟对性成熟期雄性小鼠生长发育有抑制作用,且突破了血睾屏障和血-附睾屏障进而对生殖系统产生影响。

Effect of Fluoride on Growth and Fluoride Content of Gonads in Male Mice

To explore the effect of fluoride on growth and fluoride content of gonads in male mice with sexual maturation,40 healthy male Kunming mice were distributed into 4 groups randomly including the control group(distilled water),low dose fluoride(30 mg/L NaF in their drinking water),middle dose fluoride(70 mg/L) and high dose fluoride(150 mg/L) group.During the exposure,the healthy condition of mice was observed and the body weight of mice was recorded every week.After 49 days,blood,femur,testis,epididymis,and seminal vesicle were collected and fluoride contents were deternined.Compared with the controls,there was no significant difference in all fluoride groups(P>0.05).In high group,body weight gain was significantly decreased(P<0.05).The fluoride content of femur in all fluoride groups were significantly increased compared to control group(P<0.05).Testis and epididymis fluoride contents increased in a dose-dependent manner,and were significantly higher in 150 mg/L group than those in controls(P<0.05).There was no significant difference in blood and seminal vesicle between all fluoride groups and the control group(P>0.05).The results showed that high fluoride inhibited the growth of male mice with sexual maturation,and can permeate the blood barriers of testis and epididymis to damage reproductive system.