大通牦牛MyoD1基因3'UTR SNPs多态性及其与生长性状相关性的研究

利用PCR-SSCP对296头成年大通牦牛MyoD1基因(GenBank登录号:NC_007313)外显子3全序列及3'UTR部分序列进行SNPs筛选,并将筛选到的突变位点与牦牛五项体尺指标进行相关性分析。结果发现,在3'UTR 1976 bp处检测到1个新突变,突变由C→T的转换造成,由此产生3种基因型:CC、CT、TT。利用最小二乘分析研究了该位点不同基因型对大通牦牛体尺性状的影响。结果表明,CC基因型个体的胸围、体重显著高于CT、TT基因型个体(P＜0.05),CC、CT基因型个体的体斜长显著高于TT基因型个体(P＜0.05),3种基因型对体高及管围的影响差异不显著(P＞0.05)。

Association between Polymorphism of MyoD1 Gene and Growth Traits in Yak

The polymorphisms on complete exon 3 and partial of 3′UTR were detected in 296 Datong yaks with PCR-SSCP and association between the polymorphisms and growth traits were analyzed.There was one polymorphism site in 3′UTR,which was C→T in 1976 bp,three genotypes were caused by this mutation.The association between different genotypes of this site and yak growth traits including body height,heart girth,cannon circumference,body slanting length,body weight were analyzed by least square analysis.The results showed that yaks with CC genotype had significantly higher heart girth and body weight than individuals with CT or TT genotypes(P<0.05).Yaks with CC and CT genotypes had significantly higher body slanting length than individuals with TT genotype(P<0.05),there were no significant difference among three genotypes on body height and cannon circumference.