鸡传染性法氏囊病活疫苗免疫效果评估

本试验选用A、B、C、D 4种商品化鸡传染性法氏囊病活疫苗免疫SPF鸡,进行攻毒试验以评估不同疫苗的免疫效果。10日龄SPF鸡分组后分别用A、B、C、D 4种商品化法氏囊病活疫苗按1羽份/只剂量接种免疫,21日龄时用1000 LD₅₀剂量的法氏囊超强毒株(GD0104)进行攻毒,各组疫苗的保护率分别为100%、85%、100%、100%;35日龄时用1000 LD₅₀剂量的法氏囊超强毒株(GD0104)进行攻毒,各组疫苗的保护率分别为100%、75%、95%、100%。免疫后抗体检测显示,抗体滴度水平D＞A＞C＞B,抗体转阳速度A＞D＞C＞B,免疫应激作用A、D＞B、C。综合考虑疫苗免疫攻毒后各项指标的变化,A、D疫苗免疫效果优于其他两种活疫苗,可以有效地为SPF鸡提供保护作用。

Immune Effect Assessment of Chicken IBD Live Vaccines

SPF chickens were inoculated with four commercial IBD live vaccines named A,B,C,D respectively,to assess the immune effect of different vaccines by challenged with IBDV.10 days old SPF chickens were divided into different groups and were inoculated with A,B,C,D vaccines a feather copy each chicken,challenged with vvIBDV(GD0104) 1000 LD50 doses each chicken,the protection rate of each vaccine group that challenged at 21 days old were 100%,85%,100%,100%;the protection rate of each vaccine group that challenged at 35 days old were 100%,75%,95%,100%.Antibody tests showed that the level of antibody titers D>A>C>B;the rate of antibody turned positive: A>D>C>B;immune stress effect: A,D>B,C.Considering of the indicators change post-infection vaccination,A and D vaccines were better than the other two live vaccines,can provide effective protection for the SPF chickens.