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| 鹅大肠杆菌的分离鉴定及药敏试验 | |
| 引用本文： | 严玉霖,雷华,杨尚斌,祝常芬,蒋欢,杨素红,何诸林,李丽芝,钟义广,刘小克.鹅大肠杆菌的分离鉴定及药敏试验[J].中国畜牧兽医,2011,38(10):160-162. |
| 作者姓名： | 严玉霖 雷华 杨尚斌 祝常芬 蒋欢 杨素红 何诸林 李丽芝 钟义广 刘小克 |
| 作者单位： | 1. 云南农业大学动物科学技术学院,云南昆明,650201 2. 云南云鹅牧业有限公司,云南昆明,650300 3. 云南省大理农业学校,云南大理,671003 |
| 基金项目： | 云南省高端科技人才引进计划项目(2009CI125); 清源朗德鹅技术中心建设及标准化养殖技术示范(08N060401) |
| 摘 要： | 为了对鹅大肠杆菌的临床治疗提供指导并为制备大肠杆菌自家灭活疫苗奠定基础,采集疑似病例的病料,用麦康凯培养基分离细菌,对分离的细菌进行形态学鉴定、生化鉴定、血清型鉴定、致病性试验及药敏试验。结果显示,所分离到的菌落呈粉红色、光滑湿润、边缘整齐;革兰氏染色为阴性小杆菌,两端钝圆,多单个存在;共鉴定出15株大肠杆菌,其中4株O35型,3株O78型,3株O26型,2株O7型,1株O109型,1株O141型,1株O2型;动物试验表明所鉴定的菌株对小鼠和雏鹅均有较高的致死率;体外抑菌试验结果发现强力霉素、阿莫西林对该场的鹅致病性大肠杆菌具有较强的抑制作用。本研究为有效防制鹅大肠杆菌病提供了理论依据,对于控制规模化鹅场大肠杆菌等细菌性疾病具有重要的应用价值。 |
| 关 键 词： | 鹅 大肠杆菌 分离鉴定 药敏试验 |
| 收稿时间： | 2011-03-31 |
Separation,Identification and Drug Sensitive Test of Escherichia coli from Goose |
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| YAN Yu-lin,LEI Hua,YANG Shang-bin,ZHU Chang-fen,JIANG Huan,YANG Su-hong,HE Zhu-lin,LI Li-zhi,ZHONG Yi-guang,LIU Xiao-ke.Separation,Identification and Drug Sensitive Test of Escherichia coli from Goose[J].China Animal Husbandry & Veterinary Medicine,2011,38(10):160-162. | |
| Authors: | YAN Yu-lin LEI Hua YANG Shang-bin ZHU Chang-fen JIANG Huan YANG Su-hong HE Zhu-lin LI Li-zhi ZHONG Yi-guang LIU Xiao-ke |
| Institution: | 1. College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;2. Yunnan Goose Co. Ltd.,Kunming 650300,China;3. Yunnan Dali Agricultural School,Dali 671003,China |
| Abstract: | In order to direct the treatment of goose E.coli disease and establish the foundation of preparation for the goose E.coli inactivated vaccine.The suspected cases of the goose E.coli disease were taken and separated and cultivated in MacConkey medium.The bacteria strains were obtained,and identificated by morphological assay,biochemistry assay,serotype assay,and animal experiments.Moreover,the identified E.coli were carried out by drug susceptibility test.The results showed that the strains of E.coli were pi... |
| Keywords: | goose E coli isolation and identification drug sensitive test |
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