牛锌指蛋白312基因的克隆及序列分析

本试验采用RT-PCR和测序相结合的方法克隆了牛锌指蛋白312(ZnF312)基因,得到了1812 bp的cDNA序列,包括63 bp的5'非翻译区(1-63)、1377 bp的CDS区(64-1440)和372 bp的3'非翻译区(1441-1812)。采用生物信息学方法分析了该基因及其所编码的蛋白质的结构和功能,结果表明,ZnF312基因位于22号染色体上,编码458个氨基酸,分子质量为48.82 ku,等电点为9.419,含有6个锌指结构域(ZnF_C2H2),与人、黑猩猩、小鼠、大鼠和狗的ZnF312蛋白序列的相似性分别为97%、97%、94%、94%和95%,ZnF312蛋白亚细胞定位于细胞核的概率为95.7%,定位于线粒体的概率为4.3%。结合ZnF_C2H2在真核生物中的重要作用,推测牛ZnF312蛋白可能识别相应的靶基因,在生长发育或疾病控制中起着重要的作用。

Cloning and Sequence Analysis of Bovine ZnF312 Gene

Bovine ZnF312 gene was isolated using RT-PCR and sequencing, the results showed that its cDNA sequence was 1812 bp, including 63 bp 5'-UTR, 1377 bp CDS and 372 bp 3'-UTR. The results of bio-informatics analysis for the structure and function of ZnF312 gene and its coded protein showed that ZnF312 gene was located on chromosome 22 and coded 458 amino acid. The molecular weight and isoelectric point of ZnF312 protein were 48.82 ku and 9.419, respectively. ZnF312 protein contained six ZnF_C2H2 domains, located on many cell organs, such as cell nucleus (95.7% properties) and mitochondria (4.3% properties). According the results in this study and the function of ZnF_C2H2 domain, it was inferred that ZnF312 may be very important for growth, disease resistance and susceptibility by identify the corresponding target genes.