4个家兔品种MHC-DQA基因外显子2 Mbo Ⅱ酶切多态性分析

采用PCR-RFLP方法对4个不同品种家兔MHC-DQA基因外显子2的遗传多态性进行检测,并进行聚类分析。结果表明,在家兔MHC-DQA基因外显子2的第103 bp处表现出多态性;χ²适合性检验结果表明,Mbo Ⅱ酶切位点在齐卡大型新西兰白兔和齐卡巨型白兔群体均没有达到Hardy-Weinberg平衡状态(P＜0.05),而在齐兴肉兔和加利福尼亚兔群体均达到Hardy-Weinberg平衡状态(P＞0.05);聚类分析结果表明,齐卡大型新西兰白兔与齐卡巨型白兔亲缘关系最近。试验结果为进一步利用MHC进行抗病育种提供了分子生物学基础。

Polymorphism Analysis on Exon 2 of MHC-DQA Gene in 4 Rabbit Breeds

Genetic polymorphism on exon 2 of MHC-DQA gene was investigated in 4 rabbits breeds by PCR-RFLP method with Mbo Ⅱ enzyme, and cluster analysis was also performed. The results showed that there was a polymorphism at position 103 bp of exon 2 of MHC-DQA. The χ² tests showed that Mbo Ⅱ restriction enzyme sites were not in Hardy-Weinberg equilibrium among ZIKA large New Zealand white rabbits and ZIKA giant white rabbits(P＜0.05), while in Hardy-Weinberg equilibrium among California rabbit and Qixin rabbit(P＞0.05), cluster analysis showed that ZIKA large New Zealand white rabbits had the closest genetic relationship with ZIKA giant white rabbits. The results obtained in this study will provide a molecular biological basis for further analysis of relationship of MHC polymorphism and disease resistance in rabbit.