Study of Semen Cryopreservation for Banna Mini-pig Inbred Line |
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Authors: | ZHA Xing-qin XIAO Jing CHENG Wen-min HUO Jin-long WANG Shu-yan WANG Pei PAN Wei-rong ZENG Yang-zhi |
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Institution: | 1. Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Provice, Yunnan Agricultural University, Kunming 650201, China;
2. College of Animal Sciences and Technology, Yunnan Agricultural University, Kunming 650201, China |
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Abstract: | In this study, different concentrations of glycerol were added to semen cryoprotective extenders of Banna Mini-pig inbred line (BMI), different thawing solutions and thawing methods were studied. After thawing, the motility, the rates of plasma integrity and acrosome integrity were compared to optimize the cryopreservation method of BMI semen. The results showed that the motility, the rates of plasma integrity and acrosome integrity were significantly higher in 2% and 3% glycerol groups than those in 1%, 4% and 5% glycerol groups (P<0.05);The motility, the rates of plasma integrity and acrosome integrity were significantly higher in thawing solution Ⅰ than those in thawing solution Ⅱ, Ⅲ and Ⅳ (P<0.05); Through comparing the motility, the rates of plasma integrity and acrosome integrity, the thawing effect in 40℃ 6 s and 50℃ 6 s groups were better than that in 38℃ 30 s (P<0.05). In conclusion, the optimal semen cryoprotective method for BMI was 2% or 3% glycerol as cryoprotectant, using thawing solution Ⅰ to thaw at 40℃ 6 s or 50℃ 6 s. |
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Keywords: | BMI boar semen cryopreservation thawing solution |
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