柔嫩艾美耳球虫广西南宁株的分离及鉴定

利用从南宁市郊养鸡场球虫病鸡粪便中收集的球虫混合种卵囊感染小鸡,再应用单卵囊分离感染技术,从感染鸡盲肠中收集的卵囊分离纯化获得1株纯种球虫,经鸡体传代增殖,对该虫株的卵囊大小和卵形指数、潜在期、排卵高峰期、最短孢子化时间、寄生部位、致病性等指标进行观察和测定。结果测得该虫株卵囊的平均大小为(25.743±1.94126)μm×(21.4±1.85985)μm,平均卵型指数为1.2067±0.07;潜在期为140 h;其排卵囊峰期在第6～9天,最高峰在第7天;最短孢子化时间为19 h;寄生部位在盲肠;对两周龄的艾维茵鸡,当使用5×104的孢子化卵囊感染剂量时死亡率为7.5%。根据这些测定和观察到的指标综合鉴定该分离株球虫为柔嫩艾美耳球虫,并命名为柔嫩艾美耳球虫广西南宁株(Eimeria.tenella-GXNN),该研究结果为进一步研究本地区鸡球虫病的药物治疗和免疫预防等奠定了基础。

Separation and Identification of Eimeria tenella-GXNN Strain

The mixed coccidia oocysts were collected from local chicken dung of coccidiosis and cultivated to be sporulated. Then the sporulated coccidia oocysts were inoculated to chickens. The caecum coccidia oocysts of inoculated chickens were collected. The single-oocyst isolation technique was used to abtain pure coccidia strain from the caecum coccidia oocysts. After passage propagation through chickens the indexes such as size of oocysts, latent period, sporation time of oocysts, parasitic site were observed and detected. The results showed that the average size of oocysts was （25.743±1.94126）μm×（21.4±1.85985）μm, the oocyst index was 1.2067±0.07, the latent period was 140 h, oocyst discharged peak was the seventh day, the shortest sporlation time was 19h and the coccidium parasitized in chicken caecum. According to above observed and detected materials the separated coccidium was identified to be Eimeria tenella and it was named Eimeria tenella Guangxi Nanning strain (Eimeria tenella-GXNN). This research has set up a foundation for further studying on drug and immunity prevention of chicken coccidiosis.