| Vero E6细胞酵母双杂交cDNA文库的构建及鉴定 |
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| 引用本文： | 迟延彬,石达,朱庆贺,等.Vero E6细胞酵母双杂交cDNA文库的构建及鉴定[J].中国畜牧兽医,2014,41(3):124-127. |
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| 作者姓名： | 迟延彬 石达 朱庆贺 等 |
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| 摘 要： |
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| 收稿时间： | 2013-08-19 |
Construction and Characterization of the Yeast Two-hybrid cDNA Library of Vero E6 Cells |
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| CHI Yan-bin,SHI Da,ZHU Qing-he,CHEN Jian-fei,SHI Hong-yan,ZHANG Xin,LI Chang-long,HAN Xiao,LIU Ning,ZHAO Xin,FENG Li.Construction and Characterization of the Yeast Two-hybrid cDNA Library of Vero E6 Cells[J].China Animal Husbandry & Veterinary Medicine,2014,41(3):124-127. |
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| Authors: | CHI Yan-bin SHI Da ZHU Qing-he CHEN Jian-fei SHI Hong-yan ZHANG Xin LI Chang-long HAN Xiao LIU Ning ZHAO Xin FENG Li |
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| Institution: | （1. Division of Swine Infectious Diseases，State Key Laboratory of Veterinary Biotechnology，Harbin Veterinary Research Institute，Chinese Academy of Agricultural Sciences，Harbin 150001，China；2. College of Life Science，Northeast Agricultural University，Harbin 150030，China) |
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| Abstract: | To seek out proteins which interact with structural proteins of porcine epidemic diarrhea virus （PEDV） in Vero E6 cells and study the mechanisms of viral infection.We created a Vero E6 cDNA yeast hybrid library. The total RNA of Vero E6 was extracted using Trizol method, and double strands cDNA was synthesized by SMART technique. We created a Vero E6 cDNA library by using homologous recombination in yeast. The results showed that the titer of cDNA library was 9.7×108 CFU with the average of inserted fragments about 1.6 kb, and the recombination rate was 87%. These data indicated that the yeast two-hybrid cDNA library of Vero E6 was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PEDV. |
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