刺糖对庆大霉素致小鼠肾损伤保护作用的初步研究

本试验旨在应用庆大霉素(GM)诱导建立小鼠亚急性肾损伤的模型,初探维吾尔药刺糖对庆大霉素肾损伤的保护作用。将健康昆明系小鼠28只随机分为7组,分别为A组(125 mg/kg GM)、B组(100 mg/kg GM)、C组(80 mg/kg GM)、D组(125 mg/kg GM+刺糖)、E组(100 mg/kg GM+刺糖)、F组(80 mg/kg GM+刺糖)及G组(对照组),连续用药7 d,于第8天采血后处死,分别测定血液中非蛋白氮(NPN)含量及肾脏、肝脏、脾脏的重量。试验结果显示,D组和F组血液中非蛋白氮(NPN)含量和肾脏系数分别与A组及C组相比差异显著(P<0.05);E组血液中NPN含量与B组相比差异不显著(P>0.05),而E组与B组肾脏系数相比差异显著(P<0.05)。A、B、C 3组对小鼠的肾脏都有不同程度的损伤。试验结果表明,刺糖对125和80 mg/kg GM所致的肾损伤起到了明显的保护作用,但对100 mg/kg GM所致的肾损伤未起到明显的保护作用。

Preliminary Studies of Protective Effects of Saccharum Alhagi on Kidney Injury of Mice Induced by Gentamicin

The experiment was aimed to establish the mice model of subacute kidney injury induced by gentamycin (GM), and study the effects of saccharum alhagi which was Uighur medicine on nephrotoxicity caused by gentamicin. Twenty eight mice were divided into 7 groups, group A(125 mg/kg GM), group B(100 mg/kg GM), group C(80 mg/kg GM), group D(125 mg/kg GM+saccharum alhagi), group E(100 mg/kg GM+saccharum alhagi),group F(80 mg/kg GM+saccharum alhagi) and group G(saline), respectively, intraperitoneally injected for 7 d. Mice were sacrificed at 8 d after blood collection. The blood was measured for concentration of non-protein nitrogen (NPN) and the weight of livers, spleens and kidneys. The results showed that compared with group A and C respectively, concentration of NPN and kidney coefficients of group D and group F had significant difference(P<0.05); compared with group B, concentration of NPN of group E had no significant difference(P>0.05), but kidney coefficients had significant difference(P<0.05). Kidney damage was found in the groups A, B and C. Saccharum alhagi had palyed an extremly role in the protection of kidney injury induced by 125 and 80 mg/kg GM, but didn't play a significant role in the protection of kidney injury induced by 100 mg/kg GM.