| 猪瘟病毒E2蛋白主要抗原区编码基因的原核表达及抗原性研究 |
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| 引用本文： | 刘萍,王宏博,祁光宇,刘学荣,牟克斌.猪瘟病毒E2蛋白主要抗原区编码基因的原核表达及抗原性研究[J].中国畜牧兽医,2013,40(3):43-45. |
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| 作者姓名： | 刘萍 王宏博 祁光宇 刘学荣 牟克斌 |
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| 作者单位： | 1. 中农威特生物科技股份有限公司,甘肃兰州 730046;2. 中国农业科学院兰州畜牧与兽药研究所,甘肃兰州 730050 |
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| 摘 要： | 本试验旨在分析猪瘟病毒E2蛋白的免疫反应,应用RT-PCR方法扩增了编码猪瘟病毒兔化弱毒疫苗株C株囊膜糖蛋白E2基因主要编码区,并定向克隆到表达载体pET-30a-c(+)中,获得重组表达载体pET30a-E2,将其转化大肠杆菌Rosetta(DE3)感受态细胞,经IPTG诱导表达。SDS-PAGE和Western blottin分析结果表明,E2基因获得高效融合表达,且具有免疫反应活性。本试验为建立E2抗原的检测试剂盒奠定基础。
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| 关 键 词： | 猪瘟病毒 E2蛋白 原核表达 抗原性 |
| 收稿时间： | 2012-04-18 |
The Prokaryctic Expression of the Main Antigenic Domains of E2 Protein of Classical Swine Fever Virus and Study on its Antigenicity |
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| LIU Ping
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WANG Hong-bo
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QI Guang-yu
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LIU Xue-rong
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MU Ke-bin.The Prokaryctic Expression of the Main Antigenic Domains of E2 Protein of Classical Swine Fever Virus and Study on its Antigenicity[J].China Animal Husbandry & Veterinary Medicine,2013,40(3):43-45. |
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| Authors: | LIU Ping WANG Hong-bo QI Guang-yu LIU Xue-rong MU Ke-bin |
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| Institution: | 1. China Agriculural Veterinary Biological Science and Technology Co., Ltd., Lanzhou 730046, China;2. Lanzhou Institute of Animal & Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China |
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| Abstract: | This study was aimed to analyze antigenicity of E2 protein of classical swine fever virus (CSFV). The E2 was amplified by RT-PCR from the genomic RNA of CSFV C-strain and cloned into expression vector pET-30a-c(+) to obtain recombinant pET30a-E2. The truncated E2 protein was expressed with high level in transformed Rosetta (DE3) after induction with IPTG. The results of SDS-PAGE and Western blotting indicated that the gene cloned in downstream of pET30a-E2 was expressed in high level and the recombinant fusion protein had immunologically reactive. |
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| Keywords: | classical swine fever virus E2 protein prokaryotic expression antigenicity |
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