Isolation of granulocytes and mononuclear cells from the blood of dogs, cats, horses and cattle |
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Authors: | Weiss D J Kraemer R Schmit K |
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Institution: | Department of Veterinary Pathobiology College of Veterinary Medicine University of Minnesota St. Paul, Minnesota 55108. |
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Abstract: | A simple discontinuous Percoll density-gradient technique was adapted for isolation of granulocytes and mononuclear cells from cats, dogs, horses and cattle. Separation was accomplished at low speeds using a standard tabletop centrifuge. Cell purity was 100% for both granulocytes and mononuclear cells and cell viability exceeded 95%. Percent recovery of leukocytes ranged from 69 to 83%. |
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Keywords: | Percoll density gradient leukocyte separation |
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