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Phylogentic analysis of Anaplasma ovis strains isolated from sheep and goats using groEL and mps4 genes
Authors:Anna Psaroulaki  Dimosthenis Chochlakis  Vassilios Sandalakis  Iosif Vranakis  Ioannis Ioannou  Yannis Tselentis
Institution:aLaboratory of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine (WHO Collaborating Center), Medical School, University of Crete, 71100 Voutes, Heraklion–Crete, Greece;bVeterinary Services, Nicosia, Cyprus
Abstract:Evidence of Anaplasma spp. in goats and sheep in Cyprus has been demonstrated by previous research. Herein, further research was performed for the identification of the exact Anaplasma spp. resulting in the identification of Anaplasma ovis strains in all samples examined. We used a bioinformatics as well as a molecular approach (study of groEl and mps4 genes) in order to verify the validity of the results. All samples depicted the presence of A. ovis regardless of the host (goat or sheep).
Keywords:Anaplasma ovis  Cyprus  groEL  msp4
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