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大肠杆菌对中草药敏感性试验及其方法研究
引用本文:刘玉庆,李晔,车程川,张玉忠,高培基,颜世敢.大肠杆菌对中草药敏感性试验及其方法研究[J].中兽医医药杂志,2003,22(1):3-5.
作者姓名:刘玉庆  李晔  车程川  张玉忠  高培基  颜世敢
作者单位:1. 山东大学微生物技术国家重点实验室,山东济南,250100
2. 山东省农科院畜牧兽医研究所
基金项目:山东省科学技术发展项目资助
摘    要:用平板法、管碟稀释法检测大肠杆菌、沙门氏杆菌、芽胞杆菌对23种单味中药、复方清热解毒类中药的敏感性、管碟法结合试管法,具浓度高、准确性好和平板法显示稳定的优点,比较适合中药MIC的测定。结果表明黄芩、秦皮、黄连、大黄、连翘、金银花、夏枯草、石榴皮等具有清热解毒、泻火燥湿功用的药物抑菌效果比较明显;以三黄汤为基础方的清热解毒类复方中草药抑菌作用明显,对大肠杆菌的MIC为125-500mg/ml,复方中草药比单味MIC高。同时还观察了中草药对有益菌的影响,选用了乳杆菌SM0101、芽胞杆菌007作为对照,结果表明,不同的菌株药敏性有明显差异,这是中草药特异性治疗的基础。

关 键 词:大肠杆菌  中草药  药敏试验  试验方法
文章编号:1000-6534(2003)01-0003-03
修稿时间:2002年8月28日

Sensitivity of E.coli to Chinese medicine and its method analysis
Liu Yuqing,Li Ye,Che Chengchuan,Zhang Yuzhong,Gao Peiji,Yan Shigan.Sensitivity of E.coli to Chinese medicine and its method analysis[J].Journal of Traditional Chinese Veterinary Medicine,2003,22(1):3-5.
Authors:Liu Yuqing  Li Ye  Che Chengchuan  Zhang Yuzhong  Gao Peiji  Yan Shigan
Abstract:Susceptivity of E.coli,S.enteritidis,L.acidophilus and B.cereus to 23 single and 18 compound heat-clearing and detoxifying Chinese medicines was investigated with plate method and Tube-plate method.tube-plate method is more suitable for MIC of Chinese medicine,which possesses both strong points of tube method and plate method.Heat-clearing and detoxifying drugs,such as Baikal Skullcap Root,Cortex fraxini,Chinese Goldthread Rhizome,Medicinal Rhubarb Rhizome,Weeping Forsythia Capule,Japanese Honeysuckle Flower,Common Selfheal Fruit-spike,Punica Granatum Bark,et al showed significant bacteriostasis.Heat-clearing and detoxifying compounds based on Sanhuang decoction showed less inhibition than single Chinese medicine(MIC ranged from 125 to 500 mg/ml,13 to 75 mg/ml,respectively).These heat-clearing and detoxifying drugs showed different sensitivity to the various bacteria,and they can be used in singles or combined to treat avian colibacillosis.
Keywords:E  coli  Chinese medicine  sensitivity method analysis
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