| 薄层色谱扫描法测定宫康中水苏碱含量 |
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| 引用本文： | 苗小楼,李芸,潘虎,杨耀光,苏鹏,王瑜,焦增华.薄层色谱扫描法测定宫康中水苏碱含量[J].中兽医医药杂志,2010,29(5):53-55. |
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| 作者姓名： | 苗小楼 李芸 潘虎 杨耀光 苏鹏 王瑜 焦增华 |
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| 作者单位： | 1. 中国农业科学院兰州畜牧与兽药研究所新兽药工程重点实验室,甘肃兰州,730050
2. 甘肃中医学院 |
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| 基金项目： | 国家级事业单位基本科研业务费，兰州市科技局农业攻关及产业化 |
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| 摘 要： | 目的：采用薄层色谱扫描法测定宫康中水苏碱含量。方法：采用CAMAG TLCSCANNER 3薄层色谱扫描仪,硅胶G板,展开剂：丙酮-无水乙醇-盐酸（10∶6∶1）,改良碘化铋钾-1%三氯化铁乙醇液（5∶1）为显色剂,扫描波长λs为510 nm。结果：盐酸水苏碱在3.195-38.34μg范围内线性关系良好（r=0.997）,平均回收率为96.0%,RSD为2.0%（n=6）。结论：该法灵敏、准确,是测定宫康中水苏碱的理想方法,可用于该制剂的质量控制。
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| 关 键 词： | 宫康 水苏碱 薄层色谱扫描 含量测定 |
Determination of Stachydrine contents in Gongkang perfusion by TLC scanning |
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| MIAO Xiao-lou,PAN Hu,YANG Yao-guang,SU Peng,WANG Yu,JIAO Zeng-hua.Determination of Stachydrine contents in Gongkang perfusion by TLC scanning[J].Journal of Traditional Chinese Veterinary Medicine,2010,29(5):53-55. |
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| Authors: | MIAO Xiao-lou PAN Hu YANG Yao-guang SU Peng WANG Yu JIAO Zeng-hua |
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| Institution: | (Key Laboratory of Animal Medicine Project,Lanzhou Institute of Animal ＆ Veterinary Pharmaceutics Sciences,Chinese Academy of Agricultural Sciences,Lanzhou Gansu 730050,China) |
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| Abstract: | Object ive：To determine the contents of Stachydrine in Gongkang perfusion.Methods： The CAMAG TLC scanner 3 was selected,the detection conditions were as follows： silic-gel thin-layer,developin solvent of Acetone-alcohol Anhydrous-Hydrochloric acid（10∶6∶1）,coloration of ameliorating bismuth potassium Iodide-1%ferric chloride alcohol solutions（5∶1）,the detection wavelength was 510 nm.Results： The study showed that the contents of Stachydrine was linear within the range of 3.195 to 38.34 μg （r=0.997） and the average recovery was 96.0% with RSD of 2.0%（n=6）.Conclusion： The method is ideal for controlling quality of Gongkang perfusion for its accuracy and sensitivity. |
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| Keywords: | Gongkang perfusion Stachydrine HPLC-ELSD contents determination |
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