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| 正交试验法优选金银花中绿原酸水提工艺的研究 | |
| 引用本文： | 宋德花,李继昌,梁运霞,刘广文,杨长志,刘永.正交试验法优选金银花中绿原酸水提工艺的研究[J].中兽医医药杂志,2006,25(5):5-7. |
| 作者姓名： | 宋德花 李继昌 梁运霞 刘广文 杨长志 刘永 |
| 作者单位： | 1. 东北农业大学动物医学院,黑龙江,哈尔滨,150030;黑龙江畜牧兽医职业学院 2. 东北农业大学动物医学院,黑龙江,哈尔滨,150030 3. 黑龙江畜牧兽医职业学院 4. 黑龙江出入境检验检疫局 |
| 摘 要： | 目的：优选金银花中绿原酸水提工艺。方法：采用正交试验法，以测定金银花中绿原酸提取率作为考核指标。结果：金银花中绿原酸水提工艺的最佳提取条件为不浸泡，加水（15＋12）倍量，煎煮（1＋1）h。结论：该试验绿原酸提取率高，稳定性好。 |
| 关 键 词： | 金银花 绿原酸提取率 正交试验 高效液相色谱法 |
| 文章编号： | 1000-6354（2006）05-0005-03 |
| 收稿时间： | 2006-04-06 |
| 修稿时间： | 2006年4月6日 |
Study on the optimum water extraction process for chlorogenic acid from Lonicera japonica Thunb. by orthogonal design |
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| SONG De - hua, Li ji - chang.Study on the optimum water extraction process for chlorogenic acid from Lonicera japonica Thunb. by orthogonal design[J].Journal of Traditional Chinese Veterinary Medicine,2006,25(5):5-7. | |
| Authors: | SONG De - hua Li ji - chang |
| Institution: | College of Animal Medicine, Northeast Agricultural University, Harbin Heilongjiang 150030, China |
| Abstract: | Objective: To study the optimum water extraction process for chlorogenic acid from Lonicera japonica Thunb..Methods: Orthogonal design was used to determine the optimum extraction process with the extraction rate of chlorogenic acid as markers.Results: The optimum water extraction process for chlorogenic acid from Lonicera japonica Thunb.was that the amount of water was 15+12 times and decocting time was 1+1 hours without soaking.Conclusion: The extraction process for chlorogenic acid shows high extraction rate and good stability. |
| Keywords: | Lonicera japonica Thunb chlorogenic acid extraction rate orthogonal design HPLC |
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