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金银花提取液在鸡胚内抗新城疫病毒试验
引用本文:张瑞莉,华育平,刘娣,张钊伟,梁尧,葛铭.金银花提取液在鸡胚内抗新城疫病毒试验[J].中兽医医药杂志,2010,29(4):33-36.
作者姓名:张瑞莉  华育平  刘娣  张钊伟  梁尧  葛铭
作者单位:1. 东北林业大学博士后流动站/黑龙江省农业科学院博士后工作站,黑龙江哈尔滨,150086;东北农业大学动物医学学院
2. 东北林业大学博士后流动站/黑龙江省农业科学院博士后工作站,黑龙江哈尔滨,150086
3. 东北农业大学动物医学学院
基金项目:黑龙江省青年基金项目,黑龙江省博士后资助基金项目 
摘    要:为证明金银花抗新城疫病毒的作用效果,通过接种鸡胚方法,对病毒接种前、接种同时、接种后加药3种方式处理的鸡胚尿囊液血凝效价进行测定。结果显示,在3种不同的加药方式中,不同浓度的金银花提取液均能够降低新城疫病毒感染鸡胚尿囊液的血凝效价,其作用效果与提取液浓度呈正比例相关。结果表明,金银花提取液能够抑制新城疫病毒在鸡胚尿囊液中的增殖;金银花提取液在鸡胚内抗新城疫病毒活性与其加药方式及浓度有关。

关 键 词:金银花  新城疫病毒  鸡胚

Study on the antiviral effects of Flos Lonicerae against Newcastle disease in chick embryo
Institution:ZHANG Rui-li,HUA Yu-ping,LIU Di (Post-doctoral Worker Station of Heilongjiang Academy of Agricultural Science& Northeast Agricultural University,Harbin Heilongjiang 150086,China)
Abstract:To study the effects of Flos Lonicerae against Newcastle disease virus(NDV) in chick embryo,three medicating method of Flos Lonicerae,including before inoculating virus,simultaneous inoculation with virus as well as after inoculating virus were selected and the hemagglutination titer in allantoic fluid were determined in the study.The results displayed different concentration of Flos Lonicerae could decrease the hemagglutination titer of allantoic fluid observably.This study demonstrated that Flos Lonicerae could inhibit the multiplication of NDV in chick embryo,and the activity of Flos Lonicerae against NDV had the relationship with concentration and the way of medicating.
Keywords:Flos Lonicerae  NDV  chick embryo
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