| 正交设计法优化蒲公英中黄酮提取工艺 |
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| 引用本文： | 陈虹,叶得河,杨明,王桂荣.正交设计法优化蒲公英中黄酮提取工艺[J].中兽医医药杂志,2012,31(6):40-42. |
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| 作者姓名： | 陈虹 叶得河 杨明 王桂荣 |
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| 作者单位： | 甘肃农业大学动物医学院,甘肃兰州,730070 |
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| 基金项目： | 甘肃省技术研究与开发专项计划项目 |
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| 摘 要： | 在510 nm最大吸收光时检测正交提取蒲公英中的黄酮含量,建立正交提取工艺,对提取物进行HPLC检测,色谱柱为Eclipse×DB-C18柱(4.6 mm×250 mm,5μm),流动相为甲醇∶1%冰醋酸=60∶40(v/v),流速1.0 mL/min,检测波长254 nm,柱温30℃,进样量20μL,在此色谱条件下测定黄酮含量。结果表明,各因素影响力大小为:pH值>浸泡时间>料液比;用HPLC测定蒲公英中的黄酮含量为9.26%。
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| 关 键 词： | 蒲公英 黄酮 正交设计 |
Optimization of the extracting process for flavonoid from Herba Taraxaci by orthogonal design |
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| CHEN Hong,YE De-he,YANG Ming,WANG Gui-rong.Optimization of the extracting process for flavonoid from Herba Taraxaci by orthogonal design[J].Journal of Traditional Chinese Veterinary Medicine,2012,31(6):40-42. |
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| Authors: | CHEN Hong YE De-he YANG Ming WANG Gui-rong |
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| Institution: | (College of Veterinary Medicine, Gansu Agricultural University, Lanzhou Gansu 730070, China) |
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| Abstract: | Orthogonal extraction process for flavonoid from Herba Taraxaci was established by orthogonal test in 510 nm maximum optical absorption; Then the extract was detected by HPLC. The chromatographic condition was as follows： analytic column： Eclipse ×DB-C18 （4.6 mm × 250 mm, 5 μm）, the mobile phase was methanol 1：1% glacial acetic acid = 60：40 （v/v）, the flow rate was 1.0 mL/min, UV detection wavelength was 254 nm, column temperature was 30 ℃, aud sample size was 20 μL. The results showed that the factors influence was： pH value 〉 exlracting time 〉 solid to liquid ratio： the maximal flavonoid content in Herba Taraxaci was 9.26% detected by HPLC. |
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| Keywords: | Herba Taraxaci flavonoid orthogonal experiment |
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