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蒲公英中黄酮类化合物提取工艺优化及鉴定
引用本文:王廷璞,李一婧,马伟超,袁毅君,刘玲.蒲公英中黄酮类化合物提取工艺优化及鉴定[J].中兽医医药杂志,2011,30(2):35-38.
作者姓名:王廷璞  李一婧  马伟超  袁毅君  刘玲
作者单位:天水师范学院生命科学与化学学院,甘肃天水,741000
基金项目:2008人社部留学人员项目,天水师范学院科研项目
摘    要:通过单因素筛选和正交试验优化蒲公英总黄酮的提取工艺。结果表明,乙醇浓度为45%、料液比为1∶10、提取温度为70℃、提取时间为1 h,总黄酮提取量可达26.21 mg/g。该工艺操作简单,重现性好,总黄酮的平均回收率为96.5%。

关 键 词:蒲公英  黄酮  提取工艺

Optimization for the extracting process and determination of flavonoid compounds in Taraxacum mongolicum Hand.-Mazz.
WANGTing-pu,LI Yi-jing,MAWei-chao,YUAN Yi-jun,LIULing.Optimization for the extracting process and determination of flavonoid compounds in Taraxacum mongolicum Hand.-Mazz.[J].Journal of Traditional Chinese Veterinary Medicine,2011,30(2):35-38.
Authors:WANGTing-pu  LI Yi-jing  MAWei-chao  YUAN Yi-jun  LIULing
Institution:(College of Bioscience and Chemistry,Tianshui Normal University,Tianshui Gansu 741001,China)
Abstract:The extracting process of total flavonoids from Taraxacum mongolicum Hand.-Mazz.were optimized by single factor test and orthogonal experiments.The results showed that the highest yield rate of total flavonoids reached 26.21 mg/g with 45% of ethanol,ratio of material to solvent of 1∶10,extracting for 1 h at 70 ℃.The extracting process was simple with good reproducibility,and the average recovery rate of total flavonoids was 96.5%.
Keywords:Taraxacum mongolicum Hand  -Mazz    flavonoid  extraction process
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