| 蒽酮比色法测定油菜籽中硫代葡萄糖苷条件优化 |
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| 引用本文： | 逯玉,;谭建宁,;杨蕊娟,;袁琦,;车里莫格,;朱世海.蒽酮比色法测定油菜籽中硫代葡萄糖苷条件优化[J].中兽医医药杂志,2014(4):60-61. |
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| 作者姓名： | 逯玉 ;谭建宁 ;杨蕊娟 ;袁琦 ;车里莫格 ;朱世海 |
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| 作者单位： | [1]青海省家畜改良中心,青海西宁810016; [2]青海大学生态环境工程学院,青海西宁810016 |
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| 摘 要： | 采用蒽酮比色法测定油菜籽中的硫代葡萄糖苷含量。结果表明,蒽酮比色法测定硫代葡萄糖苷的最适宜的实验条件是水浴温度60℃,蒽酮试剂添加量9 mL,显色时间为20 min。在此条件下,测得油菜籽中硫代葡萄糖苷含量保持稳定,精确度较高,且与高效液相色谱法测定结果比较接近。
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| 关 键 词： | 蒽酮比色法 硫代葡萄糖苷 油菜籽 |
Optimization on the determination of glucosinolates in rapeseed with Anthrone colorimetry |
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| Institution: | LU Yu, TAN Jian-ning, YANG Rui-juan, YUAN Qi, Cheli Moge, ZHU Shi=hai(1.Livestock Improvement Center of Qinghai Province, Xining Qinghai 810016;2.College of Environment Ecology Engineering, Qinghai University, Xining Qinghai 810016, China) |
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| Abstract: | Anthrone colorimetry was used to determine glucosinolates in rapeseed and the testing conditions was optimized. The results showed that the optimum condition for anthrone colorimetric method was water bath at 60 ℃, adding 9 ml. of anthrone reagent and coloration for 20 min. Under these conditions, the determining results Was similar to that of HPLC with high accuracy and stability. |
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| Keywords: | anthrone colorimetry glucosinolate rapeseed |
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