| 酸性染料比色法测定红茂草中总生物碱含量 |
| |
| 引用本文： | 赵强,王廷璞,董晓宁,白斌峰,赵海福.酸性染料比色法测定红茂草中总生物碱含量[J].中兽医医药杂志,2011,13(6):36-38. |
| |
| 作者姓名： | 赵强 王廷璞 董晓宁 白斌峰 赵海福 |
| |
| 作者单位： | 1. 甘肃工业职业技术学院化工学院,甘肃天水,741025
2. 天水师范学院生命科学与化学学院 |
| |
| 基金项目： | 甘肃省中医药管理局基金资助项目 |
| |
| 摘 要： | 采用酸性染料比色法测定红茂草中总生物碱含量。以异紫定堇碱为标准品,酸性染料比色法的显色条件为：pH值4.0、波长415 nm、溴甲酚绿1.5 mL,得到标准曲线：y=0.032 x-0.033,R2=0.977 0,生物碱检测浓度在21.6-54.0 mg/mL范围内与吸收度线性关系良好,红茂草中总生物碱含量为1.386%。该方法灵敏度高、准确度好、重现性好。
|
| 关 键 词： | 酸性染料比色法 红茂草 总生物碱 |
Determination of total alkaloid in Dicranostigma leptodum（Maxim.） Fedde by acid dye colorimetry |
| |
| ZHAO Qiang.Determination of total alkaloid in Dicranostigma leptodum（Maxim.） Fedde by acid dye colorimetry[J].Journal of Traditional Chinese Veterinary Medicine,2011,13(6):36-38. |
| |
| Authors: | ZHAO Qiang |
| |
| Institution: | ZHAO Qiang(Department of Chemistry Engineering,Gansu Industry Polytechnic College,Tianshui Gansu 741025,China) |
| |
| Abstract: | The content of total alkaloid in Dicranostigma leptodum（Maxim.） Fedde（DLF） was determined by acid dye colorimetry.Using the isocorydine as standard,acid dye colorimetry conditions were as follows： pH=4.0,wavelength of 425 nm,bromocresol green liquid 1.5 mL.The standard curve was then obtained： y=0.032 x-0.033,R2=0.977 0.The concentration of alkaloid showed good linearity with its absorbability within the range of 21.6-54.0 mg/mL.The content of total alkaloid from DLF was 1.386%.The method is simple,highly sensitive and reproducible. |
| |
| Keywords: | acid dye colorimetry Dicranostigma leptodum（Maxim ） Fedde total alkaloid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
